Stahl Guillaume, Salem Samia N Ben, Chen Lifeng, Zhao Bing, Farabaugh Philip J
Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA.
Eukaryot Cell. 2004 Apr;3(2):331-8. doi: 10.1128/EC.3.2.331-338.2004.
When the yeast Saccharomyces cerevisiae shifts from rapid growth on glucose to slow growth on ethanol, it undergoes profound changes in cellular metabolism, including the destruction of most of the translational machinery. We have examined the effect of this metabolic change, termed the diauxic shift, on the frequency of translational errors. Recoding sites are mRNA sequences that increase the frequency of translational errors, providing a convenient reporter of translational accuracy. We found that the diauxic shift causes no overall change in translational accuracy but does cause a strong reduction in the frequency of one type of programmed error: Ty +1 frameshifting. Genetic data suggest that this effect may be due to changes in the relative amounts of tRNA participating in translation elongation. We discuss possible implications for expression strategies that use recoding.
当酿酒酵母从以葡萄糖为碳源的快速生长转变为以乙醇为碳源的缓慢生长时，其细胞代谢会发生深刻变化，包括大部分翻译机器的破坏。我们研究了这种被称为双相转变的代谢变化对翻译错误频率的影响。重新编码位点是增加翻译错误频率的mRNA序列，是翻译准确性的便捷报告指标。我们发现，双相转变不会导致翻译准确性的总体变化，但确实会导致一种类型的程序性错误（Ty +1移码）频率大幅降低。遗传数据表明，这种影响可能是由于参与翻译延伸的tRNA相对数量的变化所致。我们讨论了使用重新编码的表达策略的可能影响。
