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本文引用的文献

1
Genetic Control of Biochemical Reactions in Neurospora.粗糙脉孢菌中生化反应的遗传控制。
Proc Natl Acad Sci U S A. 1941 Nov 15;27(11):499-506. doi: 10.1073/pnas.27.11.499.
2
An "integrated model" of programmed ribosomal frameshifting.程序化核糖体移码的“整合模型”
Trends Biochem Sci. 2002 Sep;27(9):448-54. doi: 10.1016/s0968-0004(02)02149-7.
3
New targets for antivirals: the ribosomal A-site and the factors that interact with it.抗病毒药物的新靶点：核糖体A位点及其相互作用因子。
Virology. 2002 Aug 15;300(1):60-70. doi: 10.1006/viro.2002.1567.
4
The frameshift signal of HIV-1 involves a potential intramolecular triplex RNA structure.HIV-1的移码信号涉及一种潜在的分子内三链RNA结构。
Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5331-6. doi: 10.1073/pnas.082102199.
5
Nonsense-mediated mRNA decay in Saccharomyces cerevisiae.酿酒酵母中的无义介导的mRNA降解
Gene. 2001 Aug 22;274(1-2):15-25. doi: 10.1016/s0378-1119(01)00552-2.
6
Ty1 retrotransposition and programmed +1 ribosomal frameshifting require the integrity of the protein synthetic translocation step.Ty1逆转座和程序性+1核糖体移码需要蛋白质合成易位步骤的完整性。
Virology. 2001 Jul 20;286(1):216-24. doi: 10.1006/viro.2001.0997.
7
The case against the involvement of the NMD proteins in programmed frameshifting.关于NMD蛋白参与程序性移码的反对观点。
RNA. 2000 Dec;6(12):1687-8. doi: 10.1017/s1355838200001874.
8
The case for the involvement of the Upf3p in programmed -1 ribosomal frameshifting.Upf3p参与程序性-1核糖体移码的情况。
RNA. 2000 Dec;6(12):1685-6. doi: 10.1017/s1355838200001886.
9
Nonsense-mediated decay mutants do not affect programmed -1 frameshifting.无义介导的衰变突变体不影响程序性-1移码。
RNA. 2000 Jul;6(7):952-61. doi: 10.1017/s1355838200000443.
10
A dual-luciferase reporter system for studying recoding signals.一种用于研究重编码信号的双荧光素酶报告系统。
RNA. 1998 Apr;4(4):479-86.

一种用于研究酿酒酵母中翻译重编码的体内双荧光素酶检测系统。

An in vivo dual-luciferase assay system for studying translational recoding in the yeast Saccharomyces cerevisiae.

作者信息

Harger Jason W, Dinman Jonathan D

机构信息

Department of Molecular Genetics, Microbiology and Immunology, UMDNJ Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.

出版信息

RNA. 2003 Aug;9(8):1019-24. doi: 10.1261/rna.5930803.

DOI:10.1261/rna.5930803
PMID:12869712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1236998/
Abstract

A new in vivo assay system has been developed to study programmed frameshifting in the yeast Saccharomyces cerevisiae. Frameshift signals are inserted between the Renilla and firefly luciferase reporter genes contained in a yeast expression vector and the two activities are directly measured from cell lysates in one tube. Similar to other bicistronic reporter systems, this one allows the efficient estimation of recoding efficiency by comparison of the normalized activity ratios from each luciferase protein. The assay system has been applied to HIV-1 and L-A directed programmed -1 frameshifting and Ty1 and Ty3 directed +1 frameshifting. The assay system is amenable to high-throughput screening.

摘要

已开发出一种新的体内检测系统，用于研究酿酒酵母中的程序性移码。移码信号插入酵母表达载体中包含的海肾荧光素酶和萤火虫荧光素酶报告基因之间，两种活性可直接在一管细胞裂解物中进行测量。与其他双顺反子报告系统类似，该系统通过比较每种荧光素酶蛋白的标准化活性比率，能够有效地评估重编码效率。该检测系统已应用于HIV-1和L-A介导的程序性-1移码以及Ty1和Ty3介导的+1移码。该检测系统适用于高通量筛选。