Paul Ralf, Weiser Stefan, Amiot Nicholas C, Chan Carmen, Schirmer Tilman, Giese Bernd, Jenal Urs
Division of Molecular Microbiology, Biozentrum, University of Basel, 4056 Basel, Switzerland.
Genes Dev. 2004 Mar 15;18(6):715-27. doi: 10.1101/gad.289504.
Pole development is coordinated with the Caulobacter crescentus cell cycle by two-component signaling proteins. We show that an unusual response regulator, PleD, is required for polar differentiation and is sequestered to the cell pole only when it is activated by phosphorylation. Dynamic localization of PleD to the cell pole provides a mechanism to temporally and spatially control the signaling output of PleD during development. Targeting of PleD to the cell pole is coupled to the activation of a C-terminal guanylate cyclase domain, which catalyzes the synthesis of cyclic di-guanosine monophosphate. We propose that the local action of this novel-type guanylate cyclase might constitute a general regulatory principle in bacterial growth and development.
两极发育通过双组分信号蛋白与新月柄杆菌的细胞周期相协调。我们发现，一种不同寻常的应答调节蛋白PleD是极性分化所必需的，并且只有在被磷酸化激活时才会被隔离到细胞极。PleD在细胞极的动态定位提供了一种机制，可在发育过程中对PleD的信号输出进行时空控制。PleD定位于细胞极与C端鸟苷酸环化酶结构域的激活相关联，该结构域催化环二鸟苷单磷酸的合成。我们提出，这种新型鸟苷酸环化酶的局部作用可能构成细菌生长和发育中的一种普遍调控原则。
