Paul Ralf, Jaeger Tina, Abel Sören, Wiederkehr Irene, Folcher Marc, Biondi Emanuele G, Laub Michael T, Jenal Urs
Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland.
Cell. 2008 May 2;133(3):452-61. doi: 10.1016/j.cell.2008.02.045.
The two-component phosphorylation network is of critical importance for bacterial growth and physiology. Here, we address plasticity and interconnection of distinct signal transduction pathways within this network. In Caulobacter crescentus antagonistic activities of the PleC phosphatase and DivJ kinase localized at opposite cell poles control the phosphorylation state and subcellular localization of the cell fate determinator protein DivK. We show that DivK functions as an allosteric regulator that switches PleC from a phosphatase into an autokinase state and thereby mediates a cyclic di-GMP-dependent morphogenetic program. Through allosteric activation of the DivJ autokinase, DivK also stimulates its own phosphorylation and polar localization. These data suggest that DivK is the central effector of an integrated circuit that operates via spatially organized feedback loops to control asymmetry and cell fate determination in C. crescentus. Thus, single domain response regulators can facilitate crosstalk, feedback control, and long-range communication among members of the two-component network.
双组分磷酸化网络对于细菌的生长和生理机能至关重要。在此，我们探讨该网络内不同信号转导途径的可塑性和相互联系。在新月柄杆菌中，位于相对细胞极的PleC磷酸酶和DivJ激酶的拮抗活性控制着细胞命运决定蛋白DivK的磷酸化状态和亚细胞定位。我们发现DivK作为一种变构调节因子，可将PleC从磷酸酶状态转变为自身激酶状态，从而介导一个依赖环二鸟苷酸的形态发生程序。通过变构激活DivJ自身激酶，DivK还能刺激自身的磷酸化和极性定位。这些数据表明，DivK是一个集成电路的核心效应器，该集成电路通过空间组织的反馈回路来控制新月柄杆菌中的不对称性和细胞命运决定。因此，单结构域应答调节因子可促进双组分网络成员之间的串扰、反馈控制和远程通信。
