Suwazono Yasushi, Okubo Yasushi, Kobayashi Etsuko, Miura Katsuyuki, Morikawa Yuko, Ishizaki Masao, Kido Teruhiko, Nakagawa Hideaki, Nogawa Koji
Department of Occupational and Environmental Medicine, Graduate School of Medicine, Chiba University, Chuoku, Chiba, Japan.
J Hypertens. 2004 Mar;22(3):493-500. doi: 10.1097/00004872-200403000-00011.
To examine the association between hypertension and the C825T polymorphism in the G-protein beta 3 subunit gene in Japanese workers.
This study used logistic regression analysis and multiple regression analyses to investigate whether the C825T polymorphism was associated independently with hypertension or blood pressure when factors such as age, body mass index, blood chemistry and lifestyle were taken into consideration. The target subjects were 1452 male and 1169 female workers selected from 3834 male and 2591 female workers in a single company. Hypertension was defined as a systolic blood pressure > or = 140 mmHg and/or diastolic blood pressure > or = 90 mmHg, or taking antihypertensive medication. The power of the study was estimated as 83% for males and 41% for females based on allelic frequencies in Caucasians.
Genotype distributions for C825T in hypertensive males (CC = 58, CT = 135, TT = 63) and females (CC = 20, CT = 36, TT = 20) were not significantly different from normotensive males (CC = 300, CT = 614, TT = 282) or females (CC = 274, CT = 602, TT = 217), respectively. Allele distributions were not significantly different in either sex. Multiple logistic regression analysis showed that genotype was not associated significantly with hypertension, whereas there was a significant relationship between hypertension and age, family history of hypertension, body mass index, hematocrit, platelet count, gamma-glutamyl transpeptidase (gamma-GTP) and uric acid. Data analysis using one-way analysis of variance and multiple regression showed that the C825T allele had no significant influence on either systolic, diastolic or mean blood pressure.
This study indicates that the C825T polymorphism is not a significant factor for hypertension or blood pressures in Japanese people. Targeting of this polymorphism is therefore unlikely to be beneficial when attempting to prevent hypertension in the general Japanese population.
研究日本工人中高血压与G蛋白β3亚基基因C825T多态性之间的关联。
本研究采用逻辑回归分析和多元回归分析，在考虑年龄、体重指数、血液生化指标和生活方式等因素的情况下，调查C825T多态性是否独立与高血压或血压相关。研究对象为从一家公司的3834名男性和2591名女性工人中选取的1452名男性和1169名女性工人。高血压定义为收缩压≥140 mmHg和/或舒张压≥90 mmHg，或正在服用抗高血压药物。根据白种人的等位基因频率，该研究的检验效能估计男性为83%，女性为41%。
高血压男性（CC = 58，CT = 135，TT = 63）和女性（CC = 20，CT = 36，TT = 20）中C825T的基因型分布与血压正常的男性（CC = 300，CT = 614，TT = 282）或女性（CC = 274，CT = 602，TT = 217）相比，无显著差异。等位基因分布在两性中均无显著差异。多元逻辑回归分析显示，基因型与高血压无显著关联，而高血压与年龄、高血压家族史、体重指数、血细胞比容、血小板计数、γ-谷氨酰转肽酶（γ-GTP）和尿酸之间存在显著关系。使用单因素方差分析和多元回归的数据分析表明，C825T等位基因对收缩压、舒张压或平均血压均无显著影响。
本研究表明，C825T多态性不是日本人患高血压或血压异常的重要因素。因此，针对这一多态性进行干预，在预防普通日本人群高血压方面不太可能有益。
