Lecchi M, Marguerat A, Ionescu A, Pelizzone M, Renaud P, Sommerhalder J, Safran A B, Tribollet E, Bertrand D
Department of Physiology, Faculty of Medicine, CH-1211, CMU 1, rue M. Servet, Geneva 4, Switzerland.
Neuroreport. 2004 Feb 9;15(2):307-11. doi: 10.1097/00001756-200402090-00019.
We have examined the properties of nicotinic acetylcholine receptors in embryonic chick retinal ganglion cells. Ganglion cells, identified according to morphological and physiological criteria, displayed spontaneous or induced action potentials. In 94/99 cells acetylcholine pulses evoked responses. In current clamp mode, acetylcholine provoked membrane depolarization and triggered action potentials. Under voltage clamp conditions, acetylcholine evoked inward currents that were readily blocked by d-tubocurarine. Antagonists specific for homomeric (alpha-bungarotoxin) and heteromeric (dihydro-beta-erythroidine) receptors revealed that ganglion cells express multiple functional receptor subtypes. These findings demonstrate that ACh modulates the electrical activity of these cells and is likely to mediate synaptic transmission. The presence of multiple receptor subtypes may contribute to processing and transmission of information in the retina.
我们研究了胚胎期鸡视网膜神经节细胞中烟碱型乙酰胆碱受体的特性。根据形态学和生理学标准鉴定的神经节细胞显示出自发或诱发的动作电位。在99个细胞中有94个细胞对乙酰胆碱脉冲产生反应。在电流钳模式下，乙酰胆碱引起膜去极化并触发动作电位。在电压钳条件下，乙酰胆碱诱发内向电流，该电流很容易被d -筒箭毒碱阻断。对同聚体（α-银环蛇毒素）和异聚体（二氢-β-刺桐碱）受体特异的拮抗剂表明，神经节细胞表达多种功能性受体亚型。这些发现表明乙酰胆碱调节这些细胞的电活动，并且可能介导突触传递。多种受体亚型的存在可能有助于视网膜中信息的处理和传递。
