Feller M B, Wellis D P, Stellwagen D, Werblin F S, Shatz C J
Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, 94720-3200, USA.
Science. 1996 May 24;272(5265):1182-7. doi: 10.1126/science.272.5265.1182.
Highly correlated neural activity in the form of spontaneous waves of action potentials is present in the developing retina weeks before vision. Optical imaging revealed that these waves consist of spatially restricted domains of activity that form a mosaic pattern over the entire retinal ganglion cell layer. Whole-cell recordings indicate that wave generation requires synaptic activation of neuronal nicotinic acetylcholine receptors on ganglion cells. The only cholinergic cells in these immature retinas are a uniformly distributed bistratified population of amacrine cells, as assessed by antibodies to choline acetyltransferase. The results indicate that the major source of synaptic input to retinal ganglion cells is a system of cholinergic amacrine cells, whose activity is required for wave propagation in the developing retina.
在视觉出现前数周，发育中的视网膜中就存在以动作电位自发波形式高度相关的神经活动。光学成像显示，这些波由空间受限的活动区域组成，在整个视网膜神经节细胞层形成镶嵌图案。全细胞记录表明，波的产生需要神经节细胞上神经元烟碱型乙酰胆碱受体的突触激活。通过针对胆碱乙酰转移酶的抗体评估，这些未成熟视网膜中唯一的胆碱能细胞是均匀分布的双分层无长突细胞群体。结果表明，视网膜神经节细胞突触输入的主要来源是胆碱能无长突细胞系统，其活动是发育中视网膜波传播所必需的。
