Matseliukh B P, Lutchenko V A, Polishchuk L V
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mikrobiol Z. 2003 Nov-Dec;65(6):24-30.
Landomycin-deficient mutants (24) were obtained under the effect of nitrosoguanidine on the strains 3-1 and RSp2 of Streptomyces globisporus 1912, high active procedures of polyketide antibiotic landomycin E. Two strains of these mutants produced red and one strain--yellow pigments. The absorption spectra of the pigments purified by means of thin layer chromatography were characterized by three maxima of absorption of acetone 446-448, 472-474 and 501-507 nm resembling the same characteristic of lycopene. The Rf values of isolated pigments and lycopene do not differ between themselves. The corn medium is more optimal for lycopene synthesis by strains of S. globisporus 1912 in comparison with soy one. The highly active mutants synthesized 2 mg of lycopene per 1 g of dry biomass. High frequency of spontaneous mutability of lycopene production of isolated strains can be explained by the localization of gene cluster for carotenoid biosynthesis on the one end of linear chromosome, where genetic rearrangements often take place (amplifications, deletions, recombination) because of the presence of terminal inverter DNA repetitions.
在亚硝基胍作用下，从球形孢链霉菌1912菌株3-1和RSp2中获得了缺乏兰多霉素的突变体（24），这两种菌株是聚酮类抗生素兰多霉素E的高活性生产菌株。这些突变体中有两株产生红色色素，一株产生黄色色素。通过薄层色谱法纯化的色素的吸收光谱具有三个吸收最大值，分别为丙酮的446 - 448、472 - 474和501 - 507 nm，类似于番茄红素的相同特征。分离出的色素和番茄红素的Rf值彼此无差异。与大豆培养基相比，玉米培养基更适合球形孢链霉菌1912菌株合成番茄红素。高活性突变体每1克干生物量可合成2毫克番茄红素。分离菌株番茄红素产生的自发突变频率较高，这可以通过类胡萝卜素生物合成基因簇位于线性染色体一端来解释，由于存在末端反向重复DNA，该区域经常发生基因重排（扩增、缺失、重组）。
