Matkowski Adam
Department of Pharmaceutical Biology and Botany, Medical University in Wrocław, Al. Jana Kochanowskiego 10, 51-601 Wrocław, Poland.
J Plant Physiol. 2004 Mar;161(3):343-6. doi: 10.1078/0176-1617-01145.
Pueraria lobata (Wild.) Ohwi is a medicinal plant producing large amounts of isoflavonoid glycosides. Here, the ability of in vitro callus cultures to synthesize isoflavonoids was tested. Callus cultures have been initiated from different explants of in vitro germinated plants using modified MS medium. Roots, leaves and stem segments were the best sources of callus tissue. The isoflavonoid profile and content was determined by means of chromatographic methods. Callus from all organs contained isoflavonoid aglycones: genistein and daidzein and daidzein glycosides: daidzin, puerarin and 3'-methoxypuerarin. The differences between each kind of explant were observed in both the total amount of isoflavonoids and in the proportion of individual compounds. The highest content was in root callus, followed by leaf- and stem callus.
野葛是一种能产生大量异黄酮苷的药用植物。在此，对体外愈伤组织培养物合成异黄酮的能力进行了测试。使用改良的MS培养基从体外萌发植物的不同外植体诱导出了愈伤组织培养物。根、叶和茎段是愈伤组织的最佳来源。通过色谱方法测定了异黄酮的谱图和含量。来自所有器官的愈伤组织都含有异黄酮苷元：染料木黄酮和大豆苷元，以及大豆苷元糖苷：大豆苷、葛根素和3'-甲氧基葛根素。在异黄酮总量和各化合物比例方面均观察到了每种外植体之间的差异。含量最高的是根愈伤组织，其次是叶愈伤组织和茎愈伤组织。
