Mun Song-Chol, Mun Gwan-Sim
Department of Pharmacy, Pyongyang Medical College, Kim Il Sung University, Pyongyang, Democratic People's Republic of Korea.
Department of Medical Plant Resources, Institute of Pharmaceutics, Academy of Medicine Sciences, Pyongyang, Democratic People's Republic of Korea.
J Food Drug Anal. 2015 Sep;23(3):538-544. doi: 10.1016/j.jfda.2015.04.003. Epub 2015 May 18.
Four isoflavonoids were isolated from stems of Pueraria lobata (Willd.) Ohwi growing in Democratic People's Republic of Korea and identified as daidzein (1), genistin (2), daidzin (3), and puerarin (4), structures, which were elucidated by means of spectroscopic analysis. Isoflavonoids were isolated using silica gel chromatography and purified with organic solvents. Isoflavonoid contents in P. lobata were determined using reliable high-performance liquid chromatography. The results indicated that the contents of puerarin and genistin in the roots are higher than those in the stems (6.19% and 0.04% vs. 1.15% and 0.02%), whereas the stems have higher contents of daidzin and daidzein than the roots (3.17% and 0.06% vs. 1.72% and 0.05%). Accordingly, the root part of the plant is useful for the isolation of puerarin and the stem part for daidzin. This study suggests that the stem of P. lobata is useful as an alternative source of puerarin, daidzin, genistin, and daidzein. In addition, collection of the stem will not sacrifice the plant and thus is beneficial to the natural ecosystems.
从生长在朝鲜民主主义人民共和国的野葛茎中分离出四种异黄酮，经光谱分析鉴定为大豆苷元（1）、染料木苷（2）、大豆苷（3）和葛根素（4）。异黄酮采用硅胶柱色谱法分离，并用有机溶剂纯化。采用可靠的高效液相色谱法测定野葛中的异黄酮含量。结果表明，根中葛根素和染料木苷的含量高于茎（分别为6.19%和0.04%，对比茎中的1.15%和0.02%），而茎中大豆苷和大豆苷元的含量高于根（分别为3.17%和0.06%，对比根中的1.72%和0.05%）。因此，该植物的根部分有利于葛根素的分离，茎部分有利于大豆苷的分离。本研究表明，野葛茎可作为葛根素、大豆苷、染料木苷和大豆苷元的替代来源。此外，采集茎不会牺牲植物，因此有利于自然生态系统。
