Williams Susan M, Reed Willie M, Bacon Larry D, Fadly Aly M
U.S. Department of Agriculture, Agricultural Research Service, Avian Disease and Oncology Laboratory, 3606 East Mount Hope Road, East Lansing, MI 48823, USA.
Avian Dis. 2004 Jan-Mar;48(1):61-7. doi: 10.1637/7052.
In Experiment 1, chickens from various white leghorn experimental lines were inoculated with strain ADOL-Hcl of subgroup J avian leukosis virus (ALV-J) either as embryos or at 1 day of age. At various ages, chickens were tested for ALV-J induced viremia, antibody, and packed cell volume (PCV). Also, at 4 and 10 wk of age, bursal tissues were examined for avian leukosis virus (ALV)-induced preneoplastic lesions with the methyl green-pyronine (MGP) stain. In Experiment 2, chickens harboring or lacking endogenous virus 21 (EV21) were inoculated with strain ADOL-Hcl of ALV-J at hatch. All embryo-inoculated chickens in Experiment 1 tested positive for ALV-J and lacked antibody throughout the experimental period of 30 wk and were considered viremic tolerant, regardless of line of chickens. By 10 wk of age, the incidence of ALV-J viremia in chickens inoculated with virus at hatch varied from 0 (line 0 chickens) to 97% (line 1515); no influence of ALV-J infection was noted on PCV. Results from microscopic examination of MGP-stained bursal tissues indicate that ALV-J can induce typical ALV-induced transformation in bursal follicles of white leghorn chickens. Lymphoid leukosis and hemangiomas were the most common ALV-J-induced tumors noted in chickens in Experiment 1. At termination of Experiment 2 (31 wk of age), 54% of chickens harboring EV21 were viremic tolerant compared with 5% of chickens lacking EV21 after inoculation with ALV-J at hatch. The data indicate that genetic differences among lines of white leghorn chickens, including the presence or absence of EV21, can influence response of chickens to infection with ALV-J.
在实验1中，将来自不同白来航实验品系的鸡，在胚胎期或1日龄时接种J亚群禽白血病病毒（ALV-J）的ADOL-Hcl毒株。在不同年龄阶段，检测鸡的ALV-J诱导的病毒血症、抗体和红细胞压积（PCV）。此外，在4周龄和10周龄时，用甲基绿-派洛宁（MGP）染色检查法氏囊组织中禽白血病病毒（ALV）诱导的肿瘤前病变。在实验2中，将携带或不携带内源性病毒21（EV21）的鸡在孵化时接种ALV-J的ADOL-Hcl毒株。实验1中所有经胚胎接种的鸡在30周的实验期内对ALV-J检测呈阳性，且整个实验期内均无抗体，无论鸡的品系如何，均被视为病毒血症耐受型。到10周龄时，孵化时接种病毒的鸡中ALV-J病毒血症的发生率从0（0系鸡）到97%（1515系）不等；未观察到ALV-J感染对红细胞压积有影响。对MGP染色的法氏囊组织进行显微镜检查的结果表明，ALV-J可在白来航鸡的法氏囊滤泡中诱导典型的ALV诱导的转化。淋巴细胞白血病和血管瘤是实验1中鸡身上最常见的ALV-J诱导的肿瘤。在实验2结束时（31周龄），携带EV21的鸡中有54%为病毒血症耐受型，而在孵化时接种ALV-J后，不携带EV21的鸡中这一比例为5%。数据表明，白来航鸡品系之间的遗传差异，包括EV21的有无，可影响鸡对ALV-J感染的反应。
