Lazzari Carmen Maria, Krug Luciano Percival, Quadros Onofre Francisco, Baldi Cristiano Bozzetti, Bozzetti Mary Clarisse
Federal University of Rio Grande do Sul, Porto Alegre, Brazel.
J Oral Pathol Med. 2004 May;33(5):260-3. doi: 10.1111/j.0904-2512.2004.00177.x.
Oral human papillomavirus (HPV) prevalence varies according to geographical occurrence, the type of lesion, and the method of diagnosis. The polymerase chain reaction method (PCR) appears to be more sensitive and can be easily applicable to epidemiologic studies.
To determine the frequency of HPV and its genotypes in oral lesions among patients attending a reference clinic of a university hospital.
PCR was performed to identify HPV DNA from samples of oral epithelial lesions in 80 patients. For HPV DNA amplification, MY09/MY11 consensus primers were used and specific genotypes were identified through restriction fragment of length polymorphism (RFLP) pattern.
HPV DNA was present in 11.3% of patients, and the identified genotypes were 6b, MM4 (W13B), and MM9 (PAP238A).
HPV DNA frequency in patients with oral epithelial lesions was 11.3%. The genotypes MM4 and MM9 are uncommon in oral lesions, and they are characterized as high-risk HPV types in those types of lesions.
口腔人乳头瘤病毒（HPV）的流行率因地域、病变类型及诊断方法而异。聚合酶链反应法（PCR）似乎更为灵敏，且易于应用于流行病学研究。
确定在某大学医院的一家参考诊所就诊的患者口腔病变中HPV及其基因型的频率。
对80例患者口腔上皮病变样本进行PCR以鉴定HPV DNA。使用MY09/MY11通用引物进行HPV DNA扩增，并通过限制性片段长度多态性（RFLP）模式鉴定特定基因型。
11.3%的患者存在HPV DNA，鉴定出的基因型为6b、MM4（W13B）和MM9（PAP238A）。
口腔上皮病变患者中HPV DNA频率为11.3%。MM4和MM9基因型在口腔病变中不常见，在这类病变中它们被归类为高危HPV类型。
