Berkan O, Göl M K, Içağasioğlu S, Cetnkaya O, Yildiz E, Doğan K, Günay I
Department of Cardiovascular Surgery, Cumhuriyet University Medical Faculty, Sivas, Turkey.
Eur J Vasc Endovasc Surg. 2004 May;27(5):553-8. doi: 10.1016/j.ejvs.2004.02.006.
This study tests whether sialic acid is a mediator of the lung injury following lower extremity ischemia/reperfusion (I/R). Design. Prospective randomised study.
Thirty-one Sprague-Dawley rats were randomised into four groups: group 1, aorta was exposed but not clamped; group 2, aorta clamped for 3 h, followed by 1 h of reperfusion; group 3, 50 mg/kg pentoxifylline administrated before the aorta was clamped; and group 4, 1 mg/kg dexametasone administrated before the aorta was clamped. Serial arterial blood samples for blood gas, tumor necrosis factor-alpha (TNF-alpha), and total SA (TSA) assay were obtained. The lungs were removed and histologically examined for evidence of injury.
Groups 2, 3, and 4 had significantly higher peak serum TSA concentrations compared with groups 1 (group 1 vs. 2, p=0.001; group 1 vs. 3, p=0.002; group 1 vs. 4, p=0.001). Group 3 had lower peak serum TSA concentration. Groups 2 and 4 had significantly higher peak serum TNF-alpha concentrations (p=0.0001) compared with groups 1 and 3. Group 3 had lower peak serum TNF-alpha concentration. Lower TSA and TNF-alpha levels are associated with lesser degrees of lung injury.
TSA and TNF appear during events that lead to lung injury following lower extremity I/R.
本研究旨在检测唾液酸是否为下肢缺血/再灌注（I/R）后肺损伤的介导因子。设计：前瞻性随机研究。
31只Sprague-Dawley大鼠被随机分为四组：第1组，暴露主动脉但不夹闭；第2组，夹闭主动脉3小时，然后再灌注1小时；第3组，在夹闭主动脉前给予50mg/kg己酮可可碱；第4组，在夹闭主动脉前给予1mg/kg地塞米松。采集系列动脉血样本进行血气、肿瘤坏死因子-α（TNF-α）和总唾液酸（TSA）检测。取出肺组织进行组织学检查以寻找损伤证据。
与第1组相比，第2、3和4组的血清TSA峰值浓度显著更高（第1组与第2组，p = 0.001；第1组与第3组，p = 0.002；第1组与第4组，p = 0.001）。第3组的血清TSA峰值浓度较低。与第1组和第3组相比，第2组和第4组的血清TNF-α峰值浓度显著更高（p = 0.0001）。第3组的血清TNF-α峰值浓度较低。较低的TSA和TNF-α水平与较轻程度的肺损伤相关。
TSA和TNF在下肢I/R后导致肺损伤的过程中出现。
