血管紧张素原基因六个单核苷酸多态性与原发性高血压的关系
[Relationship between six single nucleotide polymorphisms of angiotensinogen gene and essential hypertension].
作者信息
Liu Yan, Jin Wei, Jiang Zheng-wen, Zhang Kui-xing, Sheng Hai-hui, Jin Lin, Sheng Ya-yun, Huang Wei, Yu Jin-de
机构信息
Department of Cardiology, Rui Jin Hospital, Shanghai Second Medical University, Shanghai, 200025 PR China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Apr;21(2):116-9.
OBJECTIVE
To evaluate the relationship of six single nucleotide polymorphisms(SNPs) and their haplotypes of angiotensinogen(AGT) gene to essential hypertension(EH) in Chinese Han population.
METHODS
The genotypes in 185 patients with EH and 185 healthy controls were determined by the method of ABI PRISM SNaPshot Multiplex Kit using six AGT gene polymorphisms at position -217(G/A), -152(G/A), -20(A/C) and -6(G/A) in the promoter region and T174M, M235T in exon 2.
RESULTS
The distribution of AGT genotypes and alleles frequencies showed no significant difference between the group of EH and group of controls (P>0.05). However, haplotype analysis revealed that H4 haplotype frequency, which included -152A, -20C, -6A and 235T alleles, was significantly increased in the group of EH (P<0.05).
CONCLUSION
G-152A, A-20C, G-6A and M235T polymorphisms of AGT gene might play an important role in the occurrence of EH in Chinese Han population.
目的
评估血管紧张素原（AGT）基因的六个单核苷酸多态性（SNP）及其单倍型与中国汉族人群原发性高血压（EH）的关系。
方法
采用ABI PRISM SNaPshot多重试剂盒方法，对185例EH患者和185例健康对照者进行基因分型，检测AGT基因启动子区-217（G/A）、-152（G/A）、-20（A/C）和-6（G/A）位点以及外显子2中的T174M、M235T六个多态性位点。
结果
AGT基因的基因型和等位基因频率在EH组和对照组之间分布无显著差异（P>0.05）。然而，单倍型分析显示，包含-152A、-20C、-6A和235T等位基因的H4单倍型频率在EH组中显著增加（P<0.05）。
结论
AGT基因的G-152A、A-20C、G-6A和M235T多态性可能在中国汉族人群EH的发生中起重要作用。
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