Liu Bo, Liu Jihong, Wang Tao
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2004 Mar;10(3):198-201.
To investigate the method of culturing rabbit clitoral smooth muscle cells(SMC) in vitro and their biological characteristics.
Rabbit clitoral SMCs were cultured in vitro by enzymatically dispersed method, and morphological observation, cell counting and immunohistochemical staining were employed to study their biological characteristics.
Rabbit clitoral SMCs were spindle-shaped and parallelled along their longitudinal axis, and the attachment and proliferation in vitro were rapid.
Rabbit clitoral SMCs cultured in vitro can grow and maintain their steady characteristics when provided with appropriate culture condition. Clitoral SMCs cultured in vitro can serve as a cell material for researches of clitoris.
探讨体外培养兔阴蒂平滑肌细胞（SMC）的方法及其生物学特性。
采用酶消化法体外培养兔阴蒂SMC，运用形态学观察、细胞计数及免疫组织化学染色研究其生物学特性。
兔阴蒂SMC呈梭形，沿纵轴平行排列，体外贴壁及增殖迅速。
体外培养的兔阴蒂SMC在适宜培养条件下可生长并保持稳定特性。体外培养的阴蒂SMC可作为阴蒂研究的细胞材料。
