Sadeghi-Nejad H, Moreland R B, Traish A M, Nehra A, Azadzoi K M, Goldstein I
Department of Urology, Boston University School of Medicine, USA.
Int J Impot Res. 1998 Sep;10(3):165-9. doi: 10.1038/sj.ijir.3900325.
Scientific model systems for physiological evaluation and investigation of pathophysiologies in clitoral function have been limited. The aim was to develop a New Zealand White rabbit clitoral corpus cavernosum smooth muscle cell culture.
Clitoral corpus cavernosum erectile tissue was harvested and placed in culture. Clitoral smooth muscle cells which migrated out from explants were grown to confluence and subcultured. Characterizations were performed by morphological and biochemical analyses.
The cells exhibited typical morphologic characteristics of smooth muscle cells. Indirect immunofluorescence studies confirmed the presence of a-smooth muscle cell actin. Androgen and estrogen receptors were detected by specific antibodies and binding studies. The cells expressed subtypes of TGF-beta receptors. Treatment with 80 pM TGF-beta 1 24 h resulted in induction and/or increased availability of TGF-beta receptors.
An in-vitro cell culture system using rabbit clitoral smooth muscle cells was developed. These smooth muscle cells retain their biochemical and functional integrity. This in-vitro cell culture system may facilitate studies aimed at understanding the molecular basis of female sexual function.
用于阴蒂功能生理评估和病理生理学研究的科学模型系统一直很有限。本研究旨在建立新西兰白兔阴蒂海绵体平滑肌细胞培养体系。
获取阴蒂海绵体勃起组织并进行培养。从外植体迁移出来的阴蒂平滑肌细胞生长至汇合后传代培养。通过形态学和生化分析进行特性鉴定。
这些细胞呈现出平滑肌细胞典型的形态学特征。间接免疫荧光研究证实了α平滑肌肌动蛋白的存在。通过特异性抗体和结合研究检测到雄激素和雌激素受体。这些细胞表达转化生长因子-β（TGF-β）受体亚型。用80 pM TGF-β1处理24小时可诱导和/或增加TGF-β受体的可用性。
建立了一种使用兔阴蒂平滑肌细胞的体外细胞培养体系。这些平滑肌细胞保持了其生化和功能完整性。这种体外细胞培养体系可能有助于开展旨在了解女性性功能分子基础的研究。
