Am J Clin Pathol. 2004 Apr;121(4):566-73. doi: 10.1309/21DKLY2R94H192NK.
In 2000, the Canadian mycobacteriology proficiency testing program was updated and expanded to improve proficiency and standardize the main activities and testing performed in Canadian clinical mycobacteriology laboratories. The program was divided into 2 phases and included samples for acid-fast microscopy, species identification, susceptibility testing for Mycobacterium tuberculosis and nontuberculous mycobacteria, nucleic acid amplification for the detection of M. tuberculosis complex in clinical samples, and restriction fragment length polymorphism (RFLP) typing of M. tuberculosis. Results of participating laboratories are given for a 3-year period (2000-2002). Canadian laboratories exhibited a high level of proficiency in both phases of the program. Improvements were demonstrated in susceptibility testing of M. tuberculosis, and standardization was achieved with Mycobacterium avium complex susceptibility testing. Laboratories demonstrated proficiency in determining unique and identical strains of M. tuberculosis using RFLP; however, difficulties occurred with interlaboratory reproducibility.
2000年，加拿大分枝杆菌学能力验证计划进行了更新和扩展，以提高能力并规范加拿大临床分枝杆菌学实验室的主要活动和检测。该计划分为两个阶段，包括用于抗酸显微镜检查的样本、菌种鉴定、结核分枝杆菌和非结核分枝杆菌的药敏试验、临床样本中结核分枝杆菌复合群检测的核酸扩增，以及结核分枝杆菌的限制性片段长度多态性（RFLP）分型。给出了参与实验室3年期间（2000 - 2002年）的结果。加拿大实验室在该计划的两个阶段都表现出了很高的能力。在结核分枝杆菌药敏试验方面有改进，鸟分枝杆菌复合群药敏试验实现了标准化。实验室在使用RFLP确定结核分枝杆菌的独特菌株和相同菌株方面表现出能力；然而，实验室间的可重复性存在困难。
