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幼年和成年骆驼的血清蛋白电泳图谱。

Serum protein electrophoretic pattern in young and adult camels.

作者信息

Chaudhary Z I, Iqbal J, Rashid J

机构信息

Veterinary Section, Abu Dhabi Municipality PO BOX 10829, Abu Dhabi, United Arab Emirates.

出版信息

Aust Vet J. 2003 Oct;81(10):625-6. doi: 10.1111/j.1751-0813.2003.tb12508.x.

DOI:10.1111/j.1751-0813.2003.tb12508.x
PMID:15080474
Abstract

OBJECTIVE

To compare the electrophoretic pattern of serum proteins in clinically healthy adult camels (between 3 and 8 years of age) and camel calves (less than 3 months of age).

DESIGN

Laboratory analysis of serum from healthy camels.

PROCEDURE

Blood was collected from 30 healthy adult camels and 30 camel calves and the serum separated. Total protein of each serum sample was estimated by automated chemistry analyser. The proteins were fractionated by automated electrophoresis on agarose gel.

RESULTS

Serum proteins migrated on the agarose gel as one albumin, two alpha (alpha1 and alpha2-globulins), two beta (beta1 and beta2-globulins) and one gamma-globulin fractions. In adult camels the mean concentration of total protein, albumin alpha1, alpha2, beta1, beta2 and gamma-globulins was 56.8 +/- 1.5, 30.7 +/- 0.8, 2.4 +/- 0.1, 3.2 +/- 0.1, 9.7 +/- 0.3, 3.4 +/- 0.2 and 8.6 +/- 0.3 g/L, respectively. These values in calves were 49.7 +/- 1.8, 23.7 +/- 0.8, 3.2 +/- 0.2, 3.1 +/- 0.2, 14.2 +/- 0.2, 4.0 +/- 0.2 and 4.1 +/- 0.2 g/L, respectively.

CONCLUSION

The concentration of total proteins, albumin and gamma-globulins was higher (P < 0.05) in the adult camels than in camel calves. The concentrations of beta1 globulins was higher (P < 0.05) in calves as compared to adult camels.

摘要

目的

比较临床健康的成年骆驼(3至8岁)和骆驼幼崽(小于3个月龄)血清蛋白的电泳图谱。

设计

对健康骆驼血清进行实验室分析。

步骤

从30头健康成年骆驼和30头骆驼幼崽采集血液并分离血清。通过自动化学分析仪估算每个血清样本的总蛋白。蛋白质在琼脂糖凝胶上通过自动电泳进行分离。

结果

血清蛋白在琼脂糖凝胶上迁移形成一个白蛋白、两个α(α1和α2球蛋白)、两个β(β1和β2球蛋白)和一个γ球蛋白组分。成年骆驼中总蛋白、白蛋白、α1、α2、β1、β2和γ球蛋白的平均浓度分别为56.8±1.5、30.7±0.8、2.4±

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