Ahmadi-Hamedani M, Ghazvinian K, Kokhaei P, Barati M, Mahdavi A
Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Department of Animal Sciences and Food Industries, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Open Vet J. 2014;4(1):4-8. Epub 2014 Feb 2.
The aim of this study was to evaluate the influence of age and sex on the concentration of total serum protein measured by the biuret method and protein fractions determined using cellulose acetate electrophoresis in apparently healthy camels (Camelus dromedarius). Blood samples were collected from 21 camels (12 males and 9 females). The camels were further divided into two groups: 12 young camels at the age of 3 months to 2 years and 9 adult camels at the age of 3-15 years. Cellulose acetate electrophoresis of serum proteins identified five protein fractions in adult camels as young camels, these five protein fractions include albumin, α1 and α2, β and γ-globulins. In adult camels, serum levels (g/l) of total protein, albumin, α1-globulins, α2-globulins, β-globulins and γ-globulins were 80.9±3.10, 42.9±3.10, 1.3±0.22, 2.2±0.30, 11.8±0.30 and 22.6±0.20, respectively. However, in young camels, these levels (g/l) were 66.8±2.90, 40.2±2.40, 1.0±0.14, 2.6±0.30, 10.6±0.80 and 12.3±1.20, respectively. The albumin/globulin (A/G) ratio was 2.08±0.28 in adult camels and 3.77±0.53 in young ones. The mean serum concentrations of total protein and γ-globulins were significantly (P<0.05) higher and the A/G ratio was significantly lower in adult camels compared to young camels. The mean concentrations of γ-globulins were significantly higher and the A/G ratio was significantly (P<0.05) lower in females compared to male camels. The results of the present study indicate a significant effect of age and sex on the concentrations of some of the serum protein fractions in dromedary camels.
本研究的目的是评估年龄和性别对通过双缩脲法测定的骆驼（单峰驼）血清总蛋白浓度以及使用醋酸纤维素电泳法测定的蛋白组分的影响。从21头骆驼（12头雄性和9头雌性）采集血样。这些骆驼进一步分为两组：12头3个月至2岁的幼年骆驼和9头3至15岁的成年骆驼。成年骆驼和幼年骆驼血清蛋白的醋酸纤维素电泳均鉴定出五种蛋白组分，这五种蛋白组分包括白蛋白、α1和α2球蛋白、β球蛋白和γ球蛋白。成年骆驼血清中总蛋白、白蛋白、α1球蛋白、α2球蛋白、β球蛋白和γ球蛋白的水平（g/l）分别为80.9±3.10、42.9±3.10、1.3±0.22、2.2±0.30、11.8±0.30和22.6±0.20。然而，幼年骆驼中这些水平（g/l）分别为66.8±2.90、40.2±2.40、1.0±0.14、2.6±0.30、10.6±0.80和12.3±1.20。成年骆驼的白蛋白/球蛋白（A/G）比值为2.08±0.28，幼年骆驼为3.77±0.53。与幼年骆驼相比，成年骆驼血清总蛋白和γ球蛋白的平均浓度显著更高（P<0.05），而A/G比值显著更低。与雄性骆驼相比，雌性骆驼γ球蛋白的平均浓度显著更高，A/G比值显著更低（P<0.05）。本研究结果表明年龄和性别对单峰驼某些血清蛋白组分的浓度有显著影响。
