Jobling A I, Ryan J, Augusteyn R C
National Vision Research Institute of Australia, 386 Cardigan Street, Carlton, Victoria 3053.
Aust Vet J. 2003 Dec;81(12):763-5. doi: 10.1111/j.1751-0813.2003.tb14610.x.
To determine the frequency of the 107G-->C canine leukocyte adhesion deficiency (CLAD) mutation in Irish Setters from the Australian breeding population.
Genomic DNA was isolated from 87 Irish Setter blood samples and a region of the beta-2 integrin gene (ITGB2), encompassing the mutation, was amplified using real-time Polymerase Chain Reaction (PCR). Two fluorescently labelled probes were hybridised to the fragment, and fluorescence resonance energy transfer (FRET) was used to detect the 107G-->C mutation responsible for CLAD.
Three new heterozygotes were identified among 87 healthy Irish Setters from Australia. All originated from a litter sired by a known heterozygote. A total of seven heterozygotes have now been identified in 92 dogs (7.6%), representing over 90% of all major breeding stock in five Australian states. Two of the heterozygotes were recently imported adult dogs and the others were their offspring.
The frequency of the 107C allele in the Australian population of Irish Setters is lower than that in Europe. Selective breeding programs should be adopted to eliminate the mutant allele presently in two breeding lines.
确定澳大利亚繁殖种群中爱尔兰赛特犬107G→C犬白细胞黏附缺陷（CLAD）突变的频率。
从87份爱尔兰赛特犬血液样本中分离基因组DNA，使用实时聚合酶链反应（PCR）扩增包含该突变的β-2整合素基因（ITGB2）区域。将两种荧光标记的探针与该片段杂交，并使用荧光共振能量转移（FRET）检测导致CLAD的107G→C突变。
在来自澳大利亚的87只健康爱尔兰赛特犬中鉴定出3只新的杂合子。它们均来自已知杂合子所生的一窝幼犬。目前在92只犬中总共鉴定出7只杂合子（7.6%），占澳大利亚五个州所有主要繁殖种群的90%以上。其中2只杂合子是最近进口的成年犬，其他是它们的后代。
澳大利亚爱尔兰赛特犬种群中107C等位基因的频率低于欧洲。应采用选择性育种计划来消除目前存在于两个繁殖系中的突变等位基因。
