Dimova-Yaneva D, Russell D, Main M, Brooker R J, Helms P J
Department of Child Health, University of Aberdeen, Aberdeen, UK.
Clin Exp Allergy. 2004 Apr;34(4):555-8. doi: 10.1111/j.1365-2222.2004.1918.x.
It has been suggested that acute infantile bronchiolitis associated with respiratory syncytial virus (RSV) may share some pathogenic features with atopic asthma in that virus-specific IgE is produced and cysteinyl leukotrienes (cLTs) and eosinophil cationic protein (ECP) have been detected in airway secretions. ECP is a specific marker of eosinophil activation although leukotrienes can be released from a variety of cells including mast cells, eosinophils and monocytes.
To test the association between eosinophil activation and cysteinyl leukotriene production in the upper airway secretions of infants with RSV positive (RSV+ve) bronchiolitis.
Nasal lavage samples were performed in 78 infants (0.0-11.5 months) admitted to hospital with RSV+ve bronchiolitis soon after admission (0-48 h). Leukotriene C4 (LTC4) was assayed by enzyme immunoassay (EIA) and eosinophil cationic protein (ECP) by fluoroimmunoassay (FIA).
LTC4 was detectable in 51 and ECP in 57 of 78 samples with a significant positive relationship between LTC4 and ECP (r=0.557, P<0.001).
In the majority of our subjects with RSV+ve bronchiolitis ECP and LTC4 were detectable in upper airway secretions and were significantly associated with each other. In this clinical setting much of the detected LTC4 within upper airway secretions is likely to originate from the eosinophil, an observation that may have implications for clinical management and for delineation of the underlying mechanisms associated with this illness.
有人提出，与呼吸道合胞病毒（RSV）相关的急性婴儿细支气管炎可能与特应性哮喘具有一些致病特征，因为会产生病毒特异性IgE，并且在气道分泌物中已检测到半胱氨酰白三烯（cLTs）和嗜酸性粒细胞阳离子蛋白（ECP）。ECP是嗜酸性粒细胞活化的特异性标志物，尽管白三烯可从包括肥大细胞、嗜酸性粒细胞和单核细胞在内的多种细胞中释放出来。
检测RSV阳性（RSV+ve）细支气管炎婴儿上呼吸道分泌物中嗜酸性粒细胞活化与半胱氨酰白三烯产生之间的关联。
对78例（0.0 - 11.5个月）因RSV+ve细支气管炎入院的婴儿在入院后不久（0 - 48小时）进行鼻腔灌洗采样。采用酶免疫测定法（EIA）检测白三烯C4（LTC4），采用荧光免疫测定法（FIA）检测嗜酸性粒细胞阳离子蛋白（ECP）。
78份样本中，51份可检测到LTC4，57份可检测到ECP，LTC4与ECP之间存在显著正相关（r = 0.557，P < 0.001）。
在我们大多数患有RSV+ve细支气管炎的受试者中，上呼吸道分泌物中可检测到ECP和LTC4，且二者显著相关。在这种临床情况下，上呼吸道分泌物中检测到的大部分LTC4可能源自嗜酸性粒细胞，这一观察结果可能对临床管理以及阐明与该疾病相关的潜在机制具有重要意义。
