Jiang Chong-qiu, Wang Ting
Department of Chemistry, Shandong Normal University, Jinan 250014, China.
Bioorg Med Chem. 2004 May 1;12(9):2043-7. doi: 10.1016/j.bmc.2004.02.035.
The interactions between tetracycline analogues (oxytetracycline, doxycycline, methacycline) and lysozyme (LYSO) were studied by using UV-visible absorption and spectrofluorimetric method. Tetracycline analogues can make LYSO's fluorescence quenching. The quenching mechanism is static quenching. Absorption spectrum and quenching constant all support this conclusion. The binding constants of tetracycline analogues with LYSO were obtained at various temperatures. According to the Förster nonradiative energy transfer theory, we can get the distances of donators and acceptors. We also determined the main acting force between them is electrostatic gravitation according to the thermodynamic parameter.
采用紫外可见吸收光谱法和荧光光谱法研究了四环素类似物（土霉素、强力霉素、甲烯土霉素）与溶菌酶（LYSO）之间的相互作用。四环素类似物可使溶菌酶的荧光猝灭。猝灭机制为静态猝灭。吸收光谱和猝灭常数均支持该结论。在不同温度下获得了四环素类似物与溶菌酶的结合常数。根据Förster非辐射能量转移理论，得到了供体与受体之间的距离。根据热力学参数还确定了它们之间的主要作用力为静电引力。
