使用氯磺酰基噻吩甲酰三氟丙酮-铕探针荧光分光光度法测定多巴胺

Spectrofluorimetric determination of dopamine using chlorosulfonylthenoyltrifluoroacetone-europium probe.

作者信息

Zhu Xiaojing, Ge Xiaoxia, Jiang Chongqiu

机构信息

College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.

出版信息

J Fluoresc. 2007 Nov;17(6):655-61. doi: 10.1007/s10895-007-0206-0. Epub 2007 Aug 17.

DOI:10.1007/s10895-007-0206-0

PMID:17703352

Abstract

A new spectrofluorimetric method was developed for the determination of trace amounts of dopamine (DA). Using chlorosulfonylthenoyltrifluoroacetone (CTTA)-europium ion (Eu(3+)) as a fluorescent probe, in a buffer solution at pH = 10.0, DA can remarkably enhance the fluorescence intensity of the CTTA-Eu(3+) complex at lambda = 612 nm; the enhanced fluorescence intensity of Eu(3+) is proportional to the concentration of DA. Optimum conditions for the determination of DA were also investigated. The linear range and detection limit for the determination of DA were 5.0 x 10(-8) approximately 1.6 x 10(-5) mol/l and 3.2 x 10(-8) mol/l. This method is simple, practical and relatively free of interference from coexisting substances, and can be applied to assess DA in injection and human serum samples with good precision and accuracy.

摘要

开发了一种用于测定痕量多巴胺（DA）的新荧光分光光度法。使用氯磺酰基噻吩甲酰三氟丙酮（CTTA）-铕离子（Eu(3+)）作为荧光探针，在pH = 10.0的缓冲溶液中，DA可显著增强CTTA-Eu(3+)络合物在λ = 612 nm处的荧光强度；Eu(3+)增强的荧光强度与DA的浓度成正比。还研究了测定DA的最佳条件。测定DA的线性范围和检测限分别为5.0×10(-8)～1.6×10(-5) mol/L和3.2×10(-8) mol/L。该方法简单、实用，相对不受共存物质的干扰，可用于注射剂和人血清样品中DA的测定，具有良好的精密度和准确度。