Hansen Trine L, Schmidt Jens Ejbye, Angelidaki Irini, Marca Emilia, Jansen Jes la Cour, Mosbaek Hans, Christensen Thomas H
Environment & Resources DTU, Technical University of Denmark, Kongens Lyngby, Denmark.
Waste Manag. 2004;24(4):393-400. doi: 10.1016/j.wasman.2003.09.009.
A laboratory procedure is described for measuring methane potentials of organic solid waste. Triplicate reactors with 10 grams of volatile solids were incubated at 55 degrees C with 400 ml of inoculum from a thermophilic biogas plant and the methane production was followed over a 50-day period by regular measurements of methane on a gas chromatograph. The procedure involves blanks as well as cellulose controls. Methane potentials have been measured for source-separated organic household waste and for individual waste materials. The procedure has been evaluated regarding practicality, workload, detection limit, repeatability and reproducibility as well as quality control procedures. For the source-separated organic household waste a methane potential of 495 ml CH4/g VS was found. For fat and oil a lag-phase of several days was seen. The protein sample was clearly inhibited and the maximal methane potential was therefore not achieved. For paper bags, starch and glucose 63, 84 and 94% of the theoretical methane potential was achieved respectively. A detection limit of 72.5 ml CH4/g VS was calculated from the results. This is acceptable, since the methane potential of the tested waste materials was in the range of 200-500 ml CH4/g VS. The determination of methane potentials is a biological method subject to relatively large variation due to the use of non-standardized inoculum and waste heterogeneity. Therefore, procedures for addressing repeatability and reproducibility are suggested.
本文描述了一种用于测量有机固体废物甲烷潜力的实验室方法。将含有10克挥发性固体的三个重复反应器在55摄氏度下与来自嗜热沼气厂的400毫升接种物一起培养，并通过在气相色谱仪上定期测量甲烷来跟踪50天内的甲烷产量。该方法包括空白对照和纤维素对照。已测量了源头分类的有机生活垃圾和各种单独废料的甲烷潜力。对该方法在实用性、工作量、检测限、重复性和再现性以及质量控制程序方面进行了评估。对于源头分类的有机生活垃圾，发现甲烷潜力为495毫升CH₄/克挥发性固体。对于脂肪和油，观察到几天的滞后期。蛋白质样品明显受到抑制，因此未达到最大甲烷潜力。对于纸袋、淀粉和葡萄糖，分别达到了理论甲烷潜力的63%、84%和94%。根据结果计算出检测限为72.5毫升CH₄/克挥发性固体。这是可以接受的，因为测试废料的甲烷潜力在200 - 500毫升CH₄/克挥发性固体范围内。甲烷潜力的测定是一种生物学方法，由于使用了未标准化的接种物和废料的异质性，其变化相对较大。因此，建议采用解决重复性和再现性的程序。
