Ohkoshi Emika, Kamo Satoshi, Makino Mitsuko, Fujimoto Yasuo
College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
Phytochemistry. 2004 Apr;65(7):885-90. doi: 10.1016/j.phytochem.2004.02.020.
Four ent-kaurenoic acid derivatives, 2beta,16alpha,17-trihydroxy-ent-kauran-19-oic acid (1), 3beta,16alpha,17-trihydroxy-ent-kauran-19-oic acid (2), 11alpha,15beta-dihydroxy-7-O-beta-d-glucopyranosyl-ent-kaur-16-en-19-oic acid (3) and 1alpha,15beta-dihydroxy-7-O-beta-d-glucopyranosyl-ent-kaur-16-en-19-oic acid (4), were isolated together with five known compounds, 1,5-dicaffeoyl-quinic acid (5), 2-O-glucosyloxy-4-methoxy-cinnamic acid (6), phenethyl alcohol glucoside (7), phenethyl-1-O-beta-d-apiofuranosyl (1-->2) beta-d-glucopyranoside (sayaendoside) (8) and 3,6-dihydroxy-beta-ion-9-ol (9) from the 50% aqueous acetone extract of the aerial parts of Mikania hirsutissima DC. (Compositae). Compounds 1-9 were tested for their proliferative activity toward peripheral blood mononuclear cells (hPBMC); compounds 1 and 2 showed significant activity (43.8% and 36.7%, at 100 microM, respectively) on the lymphocyte.
从糙叶薇甘菊（菊科）地上部分的50%丙酮水提取物中分离得到4种对映-贝壳杉烯酸衍生物，即2β,16α,17-三羟基-对映-贝壳杉烷-19-酸（1）、3β,16α,17-三羟基-对映-贝壳杉烷-19-酸（2）、11α,15β-二羟基-7-O-β-D-吡喃葡萄糖基-对映-贝壳杉-16-烯-19-酸（3）和1α,15β-二羟基-7-O-β-D-吡喃葡萄糖基-对映-贝壳杉-16-烯-19-酸（4），同时还分离得到5种已知化合物，即1,5-二咖啡酰奎宁酸（5）、2-O-葡萄糖氧基-4-甲氧基肉桂酸（6）、苯乙醇葡萄糖苷（7）、苯乙基-1-O-β-D-芹菜呋喃糖基（1→2）β-D-吡喃葡萄糖苷（赛扬多苷）（8）和3,6-二羟基-β-紫罗兰醇（9）。对化合物1-9进行了对外周血单个核细胞（hPBMC）的增殖活性测试；化合物1和2对淋巴细胞显示出显著活性（在100μM时分别为43.8%和36.7%）。
