Department of Chemistry and Center for Innovation in Chemistry (PERCH-CIC), Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Planta Med. 2010 May;76(7):721-5. doi: 10.1055/s-0029-1240683. Epub 2009 Dec 14.
Bioassay-guided fractionation and purification of the anti-HIV-1-active MeOH extract from the leaves and twigs of Polyalthia sclerophylla led to the isolation of two new compounds, ENT-kaur-sclerodimer ( 1) and cyclotucanol 3-palmitate ( 2), along with the known ENT-kaur-16-en-19-oic acid ( 3), 15 beta-hydroxy- ENT-kaur-16-en-19-oic acid ( 4), 15 beta-acetoxy- ENT-kaur-16-en-19-oic acid ( 5), 15-oxo- ENT-kaur-16-en-19-oic acid ( 6), 16 alpha,17-dihydroxy- ENT-kauran-19-oic acid ( 7), 16 alpha-hydroxy- ENT-kauran-19-oic acid (xylopic acid) ( 8), a pseudodimer (15 alpha-hydroxy- ENT-kaur-16-en-19-oic acid/17-hydroxy- ENT-kaur-15-en-19-oic acid) ( 9), ermanin, nicotiflorin, and allantoin. Among these isolates, compound 3 was the most active in both anti-syncytium (EC (50) 13.7 microg/mL and selectivity index 3.1) and HIV-1 reverse transcriptase (IC (50) 34.1 microg/mL) assays.
从轮叶蒲桃的叶和小枝的抗 HIV-1 活性的甲醇提取物进行生物测定指导的分离和纯化，导致了两种新化合物的分离，ENT-贝壳杉二聚体（1）和环土豆醇 3-棕榈酸酯（2），以及已知的 ENT-贝壳杉-16-烯-19-酸（3），15β-羟基-ENT-贝壳杉-16-烯-19-酸（4），15β-乙酰氧基-ENT-贝壳杉-16-烯-19-酸（5），15-氧代-ENT-贝壳杉-16-烯-19-酸（6），16α，17-二羟基-ENT-贝壳杉-19-酸（7），16α-羟基-ENT-贝壳杉-19-酸（木糖醇酸）（8），假二聚体（15α-羟基-ENT-贝壳杉-16-烯-19-酸/17-羟基-ENT-贝壳杉-15-烯-19-酸）（9），埃曼宁，烟碱酸，和尿囊素。在这些分离物中，化合物 3 在抗合胞体（EC（50）13.7μg/mL 和选择性指数 3.1）和 HIV-1 逆转录酶（IC（50）34.1μg/mL）测定中最活跃。
