Lesniewska Barbara A, Messerschmidt Jürgen, Jakubowski Norbert, Hulanicki Adam
Institute for Spectrochemistry and Applied Spectroscopy, P.O. Box 101352, Dortmund D-44013, Germany.
Sci Total Environ. 2004 Apr 25;322(1-3):95-108. doi: 10.1016/j.scitotenv.2003.09.019.
The bioaccumulation of Pt, Pd and Rh by grass grown hydroponically with nutrient solutions containing these ions at elevated (38.7 mg l(-1) Pt, 21.7 mg l(-1) Pd and 7.1 mg l(-1) Rh) and medium (3.6 mg l(-1) Pt, 4.4 mg l(-1) Pd and 0.5 mg l(-1) Rh) concentrations was studied by using inductively coupled plasma sector field mass spectrometry (ICP-SFMS). The highest bioaccumulation factors were obtained for Pd and Rh in roots and for Pt in leaves. The obtained results showed that most of the studied metals were accumulated in roots, and only a small fraction was really metabolised and transported to leaves. The multi-element capability of ICP-SFMS has been exploited to study the metabolism of platinum group elements (PGEs) in cultivated plants. The species of studied metals were extracted from roots and leaves and separated into two mass fractions by ultra-filtration. The low molecular mass (<10 kDa) fractions of the root and the leaf extracts were investigated by size-exclusion chromatography (SEC) coupled on-line to ICP-SFMS. The presence of Ca, Cu, S and C in the same fractions as Pt, Pd and Rh may indicate the interaction of PGEs with phytochelatins and carbohydrates.
采用电感耦合等离子体质谱仪（ICP-SFMS）研究了在含有高浓度（38.7 mg l(-1) Pt、21.7 mg l(-1) Pd和7.1 mg l(-1) Rh）和中等浓度（3.6 mg l(-1) Pt、4.4 mg l(-1) Pd和0.5 mg l(-1) Rh）这些离子的营养液中水培生长的草对Pt、Pd和Rh的生物累积情况。Pd和Rh在根部以及Pt在叶片中的生物累积因子最高。所得结果表明，大多数研究的金属累积在根部，只有一小部分真正被代谢并转运到叶片中。利用ICP-SFMS的多元素分析能力研究了栽培植物中铂族元素（PGEs）的代谢情况。从根部和叶片中提取研究的金属物种，并通过超滤将其分离为两个质量分数。通过与ICP-SFMS在线联用的尺寸排阻色谱法（SEC）研究了根提取物和叶提取物的低分子量（<10 kDa）部分。与Pt、Pd和Rh在相同部分中存在Ca、Cu、S和C，这可能表明PGEs与植物螯合肽和碳水化合物之间存在相互作用。
