Orisakwe Orish Ebere, Akumka David Dezi, Njan Anoka Ayembe, Afonne Onyenmechi Johnson
Department of Pharmacology, College of Health Sciences, Nnamdi Azikiwe University, PMB 5001, Nnewi, Anambra State, Nigeria.
Reprod Toxicol. 2004 May;18(3):439-42. doi: 10.1016/j.reprotox.2004.02.002.
The purpose of this work was to investigate the testicular effects of Nigerian bonny light crude oil on male albino rats. Male albino rats were administered 200, 400, and 800 mg/kg body weight of bonny light crude oil dissolved in Tween 80 in their drinking water for 7 days, while the control group received Tween 80 in their drinking water only. After 7 days, the rats were sacrificed and testis excised, weighed, and processed for histological examination. Treatment with bonny light crude oil showed a dose-dependent decrease in the absolute weight of the testes, and a significant (P < 0.05) dose-dependent reduction in the epididymal sperm number (ESN). The final body weights of the animals treated with crude oil were also significantly (P < 0.05) decreased. Histological evaluation of the testes showed slight to severe degeneration or even complete absence of seminiferous tubules and necrosis of cells depending on the dose of the crude oil. This study suggests that the Nigerian bonny light crude oil is a testicular toxicant and its use as a folklore medicine may cause infertility.
本研究旨在探讨尼日利亚邦尼轻质原油对雄性白化大鼠睾丸的影响。将雄性白化大鼠分为三组，分别给予溶解于吐温80的邦尼轻质原油，剂量为200、400和800毫克/千克体重，持续7天，对照组仅给予吐温80。7天后，处死大鼠，取出睾丸，称重并进行组织学检查。结果显示，邦尼轻质原油处理后，睾丸绝对重量呈剂量依赖性下降，附睾精子数量显著（P < 0.05）减少，且原油处理组动物的最终体重也显著（P < 0.05）下降。睾丸组织学评估显示，根据原油剂量不同，睾丸出现轻度至重度变性，甚至生精小管完全缺失以及细胞坏死。本研究表明，尼日利亚邦尼轻质原油具有睾丸毒性，将其用作民间药物可能导致不育。
