Takano T, Miyauchi A, Yoshida H, Kuma K, Amino N
Department of Laboratory Medicine, Osaka University Graduate School of Medicine, D2, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
Br J Cancer. 2004 Apr 19;90(8):1600-5. doi: 10.1038/sj.bjc.6601702.
To find mRNAs whose expression differs between thyroid follicular adenomas and carcinomas, a high-throughput analysis of mRNAs in these two tumours was performed. This method, named high-throughput differential screening by serial analysis of gene expression (HDSS), combines a modified method of serial analysis of gene expression (SAGE) and real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). A total of 40 candidate tag sequences that showed extremely different expression levels between a follicular carcinoma and a follicular adenoma in the SAGE analysis were analysed by real-time quantitative RT-PCR, using RNAs from an additional four typical follicular carcinomas and adenomas. One sequence tag that represents trefoil factor 3 (TFF3) mRNA showed a clear difference in its expression level between adenomas and carcinomas. The expression levels of TFF3 mRNA in 48 follicular adenomas and 29 follicular carcinomas were measured by real-time quantitative RT-PCR using a specific probe for TFF3. They were significantly decreased in follicular carcinomas, especially in widely invasive types and those with evident metastases. These results indicate that the decreased expression of TFF3 mRNA is a marker of follicular carcinomas, especially those with a high risk of invasion or metastasis.
为了找出甲状腺滤泡性腺瘤和癌之间表达存在差异的mRNA，对这两种肿瘤中的mRNA进行了高通量分析。这种方法称为基因表达序列分析高通量差异筛选（HDSS），它结合了改良的基因表达序列分析（SAGE）方法和实时定量逆转录聚合酶链反应（RT-PCR）。通过实时定量RT-PCR，使用另外4个典型滤泡癌和腺瘤的RNA，对在SAGE分析中显示滤泡癌和滤泡性腺瘤之间表达水平差异极大的40个候选标签序列进行了分析。一个代表三叶因子3（TFF3）mRNA的序列标签在腺瘤和癌之间的表达水平存在明显差异。使用TFF3特异性探针通过实时定量RT-PCR测量了48个滤泡性腺瘤和29个滤泡癌中TFF3 mRNA的表达水平。它们在滤泡癌中显著降低，尤其是在广泛浸润型和有明显转移的肿瘤中。这些结果表明，TFF3 mRNA表达降低是滤泡癌的一个标志物，尤其是那些具有高侵袭或转移风险的滤泡癌。
