Sawada N, Kataoka K, Kondo K, Arimochi H, Fujino H, Takahashi Y, Miyoshi T, Kuwahara T, Monden Y, Ohnishi Y
Department of Oncological and Regenerative Surgery, School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan.
Br J Cancer. 2004 Apr 19;90(8):1672-8. doi: 10.1038/sj.bjc.6601746.
We examined the antitumour effect of a combination of betulinic acid (BA) and vincristine (VCR) on murine melanoma B16F10 cells in vitro and in vivo. Betulinic acid, a pentacyclic triterpene, showed a synergistic cytotoxic effect on melanoma cells by combinational use of VCR. Betulinic acid and VCR induced cell cycle arrest at different points (BA at G1 phase and VCR at G2/M phase) and caused apoptosis in B16F10 melanoma cells. In the in vivo study, VCR inhibited metastasis of tumour cells to the lung. The addition of BA to VCR augmented suppression of the experimental lung metastasis of melanoma cells in C57BL/6 mice. The number of lung nodules of more than 1 mm in diameter in mice treated with BA and VCR was less than that in mice treated with VCR alone. These results suggest that BA is an effective supplement for enhancing the chemotherapeutic effect on malignant melanoma.
我们在体外和体内研究了桦木酸（BA）与长春新碱（VCR）联合使用对小鼠黑色素瘤B16F10细胞的抗肿瘤作用。桦木酸是一种五环三萜，通过与VCR联合使用对黑色素瘤细胞显示出协同细胞毒性作用。桦木酸和VCR在不同点诱导细胞周期停滞（BA在G1期，VCR在G2/M期），并导致B16F10黑色素瘤细胞凋亡。在体内研究中，VCR抑制肿瘤细胞向肺部转移。在VCR中添加BA增强了对C57BL/6小鼠黑色素瘤细胞实验性肺转移的抑制作用。用BA和VCR处理的小鼠中直径大于1mm的肺结节数量少于仅用VCR处理的小鼠。这些结果表明，BA是增强对恶性黑色素瘤化疗效果的有效补充剂。
