Stoltenburg-Didinger G, Boegner F, Grüning W, Wagner M, Marx P, Altenkirch H
Institute of Neuropathology, Klinikum Steglitz, Free University Berlin, F.R.G.
Neurotoxicology. 1992 Spring;13(1):161-4.
Pure neurons were prepared from dorsal root ganglia (DRG) of 8 day old chick embryos (E8). The substances tested in this model were pure n-hexane, 2.5-hexanedione (2.5 HD) and methyl-ethyl-ketone (MEK). Differentiated cultures were exposed to these neurotoxins after two days in culture and then examined over a period of up to one week. For all three substances a specific neurotoxic effect could be demonstrated: (i) n-Hexane mainly altered the neurites, leading to focal swellings and in a second step to degenerative changes in glial cells; (ii) 2.5 HD had a minor effect on neurons and proved to be mainly gliatoxic; (iii) MEK primarily affected the neurons by swelling and disintegration of the cell body.
从8日龄鸡胚（E8）的背根神经节（DRG）制备纯神经元。在该模型中测试的物质为纯正己烷、2,5 -己二酮（2.5 HD）和甲基乙基酮（MEK）。分化培养物在培养两天后暴露于这些神经毒素，然后在长达一周的时间内进行检查。对于所有这三种物质，均可证明有特定的神经毒性作用：（i）正己烷主要改变神经突，导致局灶性肿胀，第二步导致神经胶质细胞发生退行性变化；（ii）2.5 HD对神经元影响较小，主要表现为对神经胶质细胞有毒性；（iii）MEK主要通过细胞体肿胀和崩解影响神经元。
