Boegner F, Grüning W, Stoltenburg-Didinger G, Marx P, Altenkirch H
Department of Neurology, Klinikum Steglitz, Free University of Berlin, F.R.G.
Neurotoxicology. 1992 Spring;13(1):151-4.
Of the metabolites of hexane, 2,5-hexanedione (2,5 HD) has the strongest neurotoxic effect. There is a wealth of experimental studies in animals showing an axonotoxic mechanism consisting of an accumulation of 10 nm neurofilaments. Only few studies deal with a possible action of 2,5-HD on Schwann cells, glia cells or both. Pure neurons, pure glia and mixed cultures prepared from dorsal root ganglia (DRG) of chick embryos were studied in this model. DRG were chosen because they constitute a linkage between the peripheral and central nervous system and provide the additional advantage of containing only few defined glial and neuronal cell types. Additionally, pure neuronal cultures of sympathetic ganglia and mixed cultures of spinal cord and brain were prepared. In cultures of the different parts of the nervous system investigated, we observed at a concentration of 0.25% 2,5-HD massive toxic alterations of glial cells, whereas neurons and neurites were virtually unaffected.
在己烷的代谢产物中，2,5 -己二酮（2,5 HD）具有最强的神经毒性作用。有大量针对动物的实验研究表明，其轴突毒性机制包括10纳米神经丝的积累。仅有少数研究涉及2,5 - HD对施万细胞、神经胶质细胞或两者可能产生的作用。在此模型中，对从鸡胚背根神经节（DRG）制备的纯神经元、纯神经胶质细胞及混合培养物进行了研究。选择DRG是因为它们构成了外周和中枢神经系统之间的联系，并且具有仅包含少数特定神经胶质细胞和神经元细胞类型的额外优势。此外，还制备了交感神经节的纯神经元培养物以及脊髓和脑的混合培养物。在所研究的神经系统不同部位的培养物中，我们观察到在浓度为0.25%的2,5 - HD作用下，神经胶质细胞出现大量毒性改变，而神经元和神经突实际上未受影响。
