经典猪瘟病毒HCLV株基因组3'非翻译区中12个核苷酸插入对RNA合成的影响。
Influence of a 12-nt insertion present in the 3' untranslated region of classical swine fever virus HCLV strain genome on RNA synthesis.
作者信息
Xiao Ming, Gao Jufang, Wang Yujing, Wang Xiaohui, Lu Wenwei, Zhen Yamei, Chen Jiakuan, Li Bo
机构信息
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, The Institute of Biodiversity Science, Fudan University, Shanghai, PR China.
出版信息
Virus Res. 2004 Jun 15;102(2):191-8. doi: 10.1016/j.virusres.2004.01.029.
The function of a 12-nt insertion 'CTTTTTTCTTTT' in the 3' untranslated region (3'UTR) of the HCLV strain, a vaccine strain derived from the Shimen strain of classical swine fever virus (CSFV), was examined in vitro. RNA synthesis increased when the 12-nt insertion was deleted from the 3'UTR of the HCLV strain. RNA synthesis also decreased when the 12-nt insertion was introduced into the 3'UTR of the CSFV Shimen, virulent strain. Therefore, the 12-nt insertion present in the 3'UTR of the HCLV strain may be a marker for avirulence. It was also found that the predicted secondary structure of the 3'UTR of the HCLV strain was more stable when the 12-nt insertion was deleted, and that the 3'UTR of Shimen strain was more unstable when the 12-nt insertion was introduced. Replacement of the 12-nt insertion 'CTTTTTTCTTTT' by another 12-nt fragment 'ATTATTATTTAT' in the 3'UTR of HCLV strain facilitated RNA synthesis and stabilized the predicted secondary structure, which was also observed in the 3'UTR of the Shimen strain.
对源自经典猪瘟病毒（CSFV）石门株的疫苗株HCLV株3'非翻译区（3'UTR）中12个核苷酸插入序列“CTTTTTTCTTTT”的功能进行了体外研究。从HCLV株的3'UTR中删除12个核苷酸插入序列后，RNA合成增加。当将12个核苷酸插入序列引入CSFV石门强毒株的3'UTR时，RNA合成也减少。因此，HCLV株3'UTR中存在的12个核苷酸插入序列可能是无毒力的一个标志物。还发现，删除12个核苷酸插入序列时，HCLV株3'UTR的预测二级结构更稳定，而引入12个核苷酸插入序列时，石门株的3'UTR更不稳定。用另一个12个核苷酸片段“ATTATTATTTAT”替换HCLV株3'UTR中的12个核苷酸插入序列“CTTTTTTCTTTT”可促进RNA合成并稳定预测的二级结构，这在石门株的3'UTR中也有观察到。
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