Herrmann Lynn M, Hötzel Isidro, Cheevers William P, On Top Kathy Pretty, Lewis Gregory S, Knowles Donald P
US Department of Agriculture, Animal Disease Research Unit, Agricultural Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630, USA.
Virus Res. 2004 Jun 15;102(2):215-20. doi: 10.1016/j.virusres.2004.02.001.
Seven new ovine progressive pneumonia virus (OPPV) field isolates were derived from colostrum and milk of 10 naturally OPPV-infected sheep from the US Sheep Experiment Station in Dubois, Idaho, USA. Sixteen sequences of the surface envelope glycoprotein (SU) from these seven Dubois OPPV field isolates and SU sequence from OPPV WLC1 were obtained, aligned with published SRLV SU sequences, and analyzed using phylogenetic analysis using parsimony (PAUP). Percent nucleotide identity in SU was greater than 95.8% among clones from individual Dubois OPPVs and ranged from 85.5 to 93.8% between different Dubois OPPV clones. SU sequences from Dubois OPPVs and WLC1 OPPV had significantly higher percent nucleotide identity to SU sequences from the North American OPPVs (85/34 and S93) than caprine-arthritis encephalitis virus (CAEVs) or MVVs. PAUP analysis also showed that SU sequences from the Dubois OPPVs and OPPV WLC1 grouped with other North American OPPVs (85/34 and S93) with a bootstrap value of 100 and formed one OPPV clade II group. In addition, Dubois and WLC1 SU amino acid sequences had significantly higher identity to SU sequences from North American OPPVs than CAEV or MVV. These data indicate that the seven new Dubois OPPV field isolates along with WLC1 OPPV are part of the OPPV clade II and are distinct from CAEVs and MVVs.
从美国爱达荷州杜波依斯市美国绵羊实验站的10只自然感染绵羊进行性肺炎病毒（OPPV）的初乳和乳汁中分离出7株新的OPPV野外分离株。获得了这7株杜波依斯OPPV野外分离株的表面包膜糖蛋白（SU）的16个序列以及OPPV WLC1的SU序列，与已发表的绵羊肺腺瘤病毒（SRLV）SU序列进行比对，并使用简约法（PAUP）进行系统发育分析。来自单个杜波依斯OPPV的克隆之间SU的核苷酸同一性百分比大于95.8%，不同杜波依斯OPPV克隆之间的核苷酸同一性百分比在85.5%至93.8%之间。杜波依斯OPPV和WLC1 OPPV的SU序列与北美OPPV（85/34和S93）的SU序列相比，其核苷酸同一性百分比显著高于山羊关节炎脑炎病毒（CAEV）或梅迪-维斯纳病毒（MVV）。PAUP分析还表明，杜波依斯OPPV和OPPV WLC1的SU序列与其他北美OPPV（85/34和S93）归为一组，自展值为100，形成了一个OPPV进化枝II组。此外，杜波依斯和WLC1的SU氨基酸序列与北美OPPV的SU序列相比，与CAEV或MVV相比具有显著更高的同一性。这些数据表明，这7株新的杜波依斯OPPV野外分离株以及WLC1 OPPV是OPPV进化枝II的一部分，并且与CAEV和MVV不同。
