Mwaengo D M, Grant R F, DeMartini J C, Carlson J O
Department of Pathology, Colorado State University, Fort Collins 80523, USA.
Virology. 1997 Nov 10;238(1):135-44. doi: 10.1006/viro.1997.8813.
Ovine lentiviruses (OvLV) resemble human immunodeficiency viruses in genomic organization, viral heterogeneity, and spectrum of cytophenotypic expression. To gain a better understanding of the relationship of North American OvLV isolates with other characterized OvLV strains, the complete DNA nucleotide sequence of the env region of a highly lytic (rapid/high) OvLV strain (85/34) was determined and compared with the sequence of amplicons within env of three other OvLV strains of varying cytophenotype and isolated from the same flock of sheep. LTR and pol regions also were compared among these strains. The env region of 85/34 was 986 codons in length and the reported nucleotide sequence showed features shared by other OvLV including heavy glycosylation and conserved and hypervariable regions within the surface membrane protein region. Phylogenetic analyses of regions within LTR, reverse transcriptase, and env grouped the four virus strains together and similar to the maedi-visna OvLV strains, including visna virus, South African ovine maedi visna virus, and EV1 (British OvLV isolate), but they were distinct from caprine arthritis encephalitis virus.
绵羊慢病毒（OvLV）在基因组结构、病毒异质性和细胞表型表达谱方面与人类免疫缺陷病毒相似。为了更好地理解北美OvLV分离株与其他已鉴定的OvLV毒株之间的关系，测定了一种高裂解性（快速/高效）OvLV毒株（85/34）env区域的完整DNA核苷酸序列，并将其与从同一羊群中分离出的另外三种具有不同细胞表型的OvLV毒株env内扩增子的序列进行了比较。还对这些毒株的LTR和pol区域进行了比较。85/34的env区域长度为986个密码子，所报道的核苷酸序列显示出与其他OvLV共有的特征，包括高度糖基化以及表面膜蛋白区域内的保守和高变区域。对LTR、逆转录酶和env区域的系统发育分析将这四种病毒毒株归为一组，并且与梅迪-维斯纳OvLV毒株相似，包括维斯纳病毒、南非绵羊梅迪-维斯纳病毒和EV1（英国OvLV分离株），但它们与山羊关节炎脑炎病毒不同。
