Snead M L, Luo W, Hsu D D, Melrose R J, Lau E C, Stenman G
Department of Basic Sciences USC, School of Dentistry, Los Angeles.
Oral Surg Oral Med Oral Pathol. 1992 Jul;74(1):64-72. doi: 10.1016/0030-4220(92)90217-e.
Instructive signals are responsible for the regulation of the expression of gene products characteristic of many cell lineages during normal development and potentially during neoplasia. The odontogenic origin of ameloblastomas is based largely on the similarity in histologic appearance between the tumor and the developing tooth organ. A pathognomonic pattern for odontogenic tissue-specific gene expression in ameloblastomas has not been previously shown. In these studies, the gene expression parameters for human ameloblastomas have been characterized with the techniques of messenger RNA phenotyping in combination with Northern and in situ hybridization analysis of messenger RNA. The results of these studies confirm that amelogenin, a gene transcribed solely by differentiated ameloblasts, was expressed by epithelial cells from human ameloblastomas. This observation suggests that the instructive signals required for ameloblast differentiation are shared during normal development and tumorigenesis of odontogenic epithelium.
在正常发育过程中以及肿瘤形成过程中，诱导信号负责调控许多细胞谱系所特有的基因产物的表达。成釉细胞瘤的牙源性起源很大程度上基于肿瘤与发育中的牙器官在组织学外观上的相似性。此前尚未显示成釉细胞瘤中牙源性组织特异性基因表达的特征性模式。在这些研究中，利用信使核糖核酸表型分析技术结合信使核糖核酸的Northern杂交和原位杂交分析，对人类成釉细胞瘤的基因表达参数进行了表征。这些研究结果证实，釉原蛋白是一种仅由分化的成釉细胞转录的基因，在人类成釉细胞瘤的上皮细胞中表达。这一观察结果表明，在牙源性上皮的正常发育和肿瘤发生过程中，成釉细胞分化所需的诱导信号是共享的。
