Fortunato Stephen J, Menon Ramkumar, Ahmed Nasar U, Bourgeois Michelle, Dildy Gary A
Perinatal Research Center, The Centennial Women's Hospital, Patterson, Nashville, TN 37203, USA.
J Perinat Med. 2004;32(2):122-5. doi: 10.1515/JPM.2004.022.
Polyhydramnios places increased tension on the fetal membranes and should conceptually be associated with an increased need for tissue remodeling to prevent premature rupture of membranes (PROM). Herein we use polyhydramnios as a model to study the association of the collagenase class of matrix metalloproteinases (MMPs) with tissue remodeling during pregnancy.
Amniotic fluids were collected by transabdominal amniocentesis from women with polyhydramnios and from women with normal amniotic fluid volume at second trimester, third trimester and at term. Fluids were assayed for MMP-1 and MMP-13 (collagenase-1 and 3) using ELISA. The concentrations in polyhydramnios were compared with subjects undergoing amniocentesis with normal amniotic fluid volume. Statistical comparisons were made using Scheffe's method for correction in multiple means comparison. P of <0.05 was considered statistically significant.
MMP-1 and MMP-13 are present in the amniotic fluid at all stages of pregnancy. The concentrations of these MMPs were significantly elevated in the polyhydramnios samples independent of gestational age.
Polyhydramnios is associated with increased amniotic fluid concentrations of MMP-1 and MMP-13 suggesting their role in tissue remodeling.
羊水过多会增加胎膜的张力，理论上应与为预防胎膜早破（PROM）而增加的组织重塑需求相关。在此，我们以羊水过多为模型，研究基质金属蛋白酶（MMPs）中的胶原酶类别与孕期组织重塑的关联。
通过经腹羊膜腔穿刺术，从孕中期、孕晚期及足月时羊水过多的女性和羊水正常的女性中采集羊水。使用酶联免疫吸附测定法（ELISA）检测羊水样本中的MMP-1和MMP-13（胶原酶-1和胶原酶-3）。将羊水过多患者的浓度与羊水正常且接受羊膜腔穿刺术的受试者进行比较。采用谢费法进行多重均值比较校正的统计分析。P值<0.05被认为具有统计学意义。
MMP-1和MMP-13在孕期各阶段的羊水中均有存在。这些MMPs的浓度在羊水过多样本中显著升高，且与孕周无关。
羊水过多与羊水中MMP-1和MMP-13浓度升高相关，提示它们在组织重塑中发挥作用。
