Kim Hong Joo, Lee Kyu Taek, Kim Eun Kyung, Sohn Tae Sung, Heo Jin Seok, Choi Seong Ho, Choi Dong Il, Lee Jong Kyun, Paik Seung Woon, Rhee Jong Chul
Division of Gastroenterology and Gastrointestinal Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
J Gastroenterol Hepatol. 2004 May;19(5):582-8. doi: 10.1111/j.1440-1746.2003.03299.x.
No information is available on the nature of the correlation between cyclooxygenase-2 (COX-2) expression and the clinicopathological features and prognosis of cholangiocarcinoma (CC). The goal of the present study was to determine the possible roles and clinical significance of COX-2 expression in CC.
We investigated the immunohistochemical expression of COX-2 in 102 patients with CC with respect to clinicopathological characteristics, namely evidence of Clonorchis sinensis infection, proliferation index (PI, assessed by Ki-67 expression), apoptotic index (AI, assessed by TUNEL stain), and microvessel density (MVD, assessed by CD34 expression). Evidence of C. sinensis infection was assessed by the microscopic examination of stools for C. sinensis ova, serological testing (ELISA), and the detection of peripheral bile duct dilations by imaging studies.
An immunohistochemical investigation demonstrated the immunolabeling of tumor cells, mainly in the cytoplasmic and perinuclear regions, in 53 (52%) of the 102 patients with CC. No significant differences were found in terms of age, sex, tumor differentiation, involvement of the resection margin, presence of lymph nodes or liver metastases, or in pTNM stage between COX-2 positive and COX-2 negative patients. However, evidence of C. sinensis infection was more common in COX-2 positive patients (P < 0.05). No significant differences were found for PI, AI, MVD, or cumulative survival between COX-2 positive and COX-2 negative patients.
Clonorchis sinensis infection is related to aberrant COX-2 expression in patients with CC. However, COX-2 expression is not related to clinical outcome in CC patients.
关于环氧化酶-2（COX-2）表达与胆管癌（CC）的临床病理特征及预后之间相关性的本质尚无相关信息。本研究的目的是确定COX-2表达在CC中的可能作用及临床意义。
我们调查了102例CC患者中COX-2的免疫组化表达情况，涉及临床病理特征，即华支睾吸虫感染证据、增殖指数（PI，通过Ki-67表达评估）、凋亡指数（AI，通过TUNEL染色评估）和微血管密度（MVD，通过CD34表达评估）。通过粪便中华支睾吸虫虫卵的显微镜检查、血清学检测（ELISA）以及影像学研究检测外周胆管扩张来评估华支睾吸虫感染证据。
免疫组化研究显示，102例CC患者中有53例（52%）的肿瘤细胞主要在细胞质和核周区域出现免疫标记。COX-2阳性和COX-2阴性患者在年龄、性别、肿瘤分化、切缘受累情况、有无淋巴结或肝转移以及pTNM分期方面均未发现显著差异。然而，华支睾吸虫感染证据在COX-2阳性患者中更为常见（P < 0.05）。COX-2阳性和COX-2阴性患者在PI、AI、MVD或累积生存率方面未发现显著差异。
华支睾吸虫感染与CC患者异常的COX-2表达有关。然而，COX-2表达与CC患者的临床结局无关。
