人类DNA连接酶I基因的结构。
Structure of the human DNA ligase I gene.
作者信息
Noguiez P, Barnes D E, Mohrenweiser H W, Lindahl T
机构信息
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK.
出版信息
Nucleic Acids Res. 1992 Aug 11;20(15):3845-50. doi: 10.1093/nar/20.15.3845.
Abstract
The gene encoding DNA ligase I, the major DNA ligase activity in proliferating mammalian cells, maps to human chromosome 19q13.2-13.3. We have determined the complete structure of the gene, which is composed of 28 exons spanning 53kb on this chromosome. The first exon is untranslated, and utilises a GC dinucleotide instead of the canonical GT splice donor. The 5' flanking region lacks a TATA box and is highly GC-rich, as is characteristic of a 'housekeeping' gene. In common with the promoters of genes encoding other DNA replication enzymes, such as DNA polymerase alpha, the 5' flanking region of the DNA ligase I gene contains recognition elements for several transcription factors which may mediate increased expression in quiescent cells in response to growth factors.
摘要
编码DNA连接酶I的基因是增殖哺乳动物细胞中的主要DNA连接酶活性基因，定位于人类染色体19q13.2 - 13.3。我们已经确定了该基因的完整结构，它由28个外显子组成，在这条染色体上跨越53kb。第一个外显子不编码，使用GC二核苷酸而非典型的GT剪接供体。5'侧翼区域缺乏TATA框，且富含GC，这是“管家”基因的特征。与编码其他DNA复制酶的基因（如DNA聚合酶α）的启动子一样，DNA连接酶I基因的5'侧翼区域含有几个转录因子的识别元件，这些元件可能介导静止细胞中因生长因子而增加的表达。
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