van Duin M, Koken M H, van den Tol J, ten Dijke P, Odijk H, Westerveld A, Bootsma D, Hoeijmakers J H
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Nucleic Acids Res. 1987 Nov 25;15(22):9195-213. doi: 10.1093/nar/15.22.9195.
In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.
本报告介绍了人类切除修复基因ERCC-1的基因组特征。该基因由10个外显子组成，分布在约15 kb的区域。通过转染实验，ERCC-1启动子被定位在转录起始位点上游+/- 170 bp的区域。该区域不存在CAAT、TATA和GC盒等经典启动子元件。此外，ERCC-1转录不受紫外线诱导。为先前报道的外显子VIII的可变剪接提供了一种可能的解释。对ERCC-1 cDNA克隆的分析揭示了差异聚腺苷酸化的发生，除了主要的1.1 kb mRNA外，还产生了3.4 kb和3.8 kb的ERCC-1转录本。ERCC-1转录本差异聚腺苷酸化的明显进化保守性表明这种RNA加工模式在ERCC-1修复功能中可能发挥作用。
