Sofo Adriano, Dichio Bartolomeo, Xiloyannis Cristos, Masia Andrea
Dipartimento di Produzione Vegetale, Università degli Studi della Basilicata, Campus Macchia Romana, 85100, Potenza, Italy.
Physiol Plant. 2004 May;121(1):58-65. doi: 10.1111/j.0031-9317.2004.00294.x.
The olive tree (Olea europaea L.) is commonly grown in the Mediterranean basin and is able to resist severe and prolonged drought. Levels of proline (PRO) and malondialdehyde (MDA), and the lipoxygenase (LOX) activity were determined in 2-year-old olive plants (cv. 'Coratina') grown in environmental conditions characterized by high temperatures and high photosynthetic photon flux density levels and gradually subjected to a controlled water deficit for 20 days. Before and during the experimental period, leaf and root samples were collected and analysed for PRO and MDA. The levels of PRO increased in parallel with the severity of drought stress in both leaves and roots. Significant increases of LOX activity and MDA content were also observed during the progressive increment of drought stress in both leaf and root tissues. Measurements of transpiration and photosynthetic rate, stomatal conductance and substomatal CO(2) concentration were carried out during the experiment. The accumulation of PRO indicates a possible role of PRO in drought tolerance. The increases of MDA content and LOX activity show that the water deficit is associated with lipid peroxidation mechanisms.
油橄榄树（油橄榄）通常生长在地中海盆地，能够抵御严重且持久的干旱。对种植于高温和高光量子通量密度环境条件下的2年生油橄榄植株（品种‘科拉蒂纳’）进行研究，这些植株逐渐遭受20天的可控水分亏缺，期间测定了脯氨酸（PRO）和丙二醛（MDA）含量以及脂氧合酶（LOX）活性。在实验期之前和期间，采集叶片和根系样本并分析PRO和MDA含量。叶片和根系中PRO含量均随干旱胁迫严重程度平行增加。在叶片和根系组织干旱胁迫逐渐加重过程中，还观察到LOX活性和MDA含量显著增加。实验期间测定了蒸腾速率、光合速率、气孔导度和胞间CO₂浓度。PRO的积累表明PRO在耐旱性方面可能发挥作用。MDA含量和LOX活性的增加表明水分亏缺与脂质过氧化机制有关。
