Zhang Yuntong, Zhang Ting, Zheng Yongjie, Wang Jun, Luo Chenglin, Li Yuhua, Liu Xinliang
State Key Laboratory of Tree Genetics and Breeding, Beijing Forestry University, Beijing 100083, China.
Camphor Engineering and Technology Research Centre of National Forestry and Grassland Administration, Jiangxi Academy of Forestry, Nanchang 330032, China.
Int J Mol Sci. 2025 Jul 27;26(15):7264. doi: 10.3390/ijms26157264.
is an ecologically and economically significant species, highly valued for its essential oil production and environmental benefits. Although a tissue culture system has been established for , large-scale propagation remains limited due to the inconsistent formation of adventitious roots (ARs). This study investigated AR formation from callus tissue, focusing on associated physiological changes and gene expression dynamics. During AR induction, contents of soluble sugars and proteins decreased, alongside reduced activities of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO). Levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) decreased significantly throughout AR formation. Zeatin riboside (ZR) levels initially declined and then rose, whereas gibberellic acid (GA) levels displayed the opposite trend. Comparative transcriptomic and temporal expression analyses identified differentially expressed genes (DEGs), which were grouped into four distinct expression patterns. KEGG pathway enrichment indicated that 67 DEGs are involved in plant hormone signaling pathways and that 38 DEGs are involved in the starch and sucrose metabolism pathway. Additionally, protein-protein interaction network (PPI) analysis revealed ten key regulatory genes, which are mainly involved in auxin, cytokinin, GA, ABA, and ethylene signaling pathways. The reliability of the transcriptome data was further validated by quantitative real-time PCR. Overall, this study provides new insights into the physiological and molecular mechanisms underlying AR formation in and offers valuable guidance for optimizing tissue culture systems.
是一种具有生态和经济意义的物种，因其精油生产和环境效益而备受重视。尽管已经建立了该物种的组织培养体系，但由于不定根形成不一致，大规模繁殖仍然受到限制。本研究调查了愈伤组织中不定根的形成，重点关注相关的生理变化和基因表达动态。在不定根诱导过程中，可溶性糖和蛋白质含量下降，同时抗氧化酶活性降低，包括超氧化物歧化酶（SOD）、过氧化物酶（POD）和多酚氧化酶（PPO）。在整个不定根形成过程中，吲哚-3-乙酸（IAA）和脱落酸（ABA）水平显著下降。玉米素核苷（ZR）水平先下降后上升，而赤霉素（GA）水平则呈现相反趋势。比较转录组学和时间表达分析确定了差异表达基因（DEG），这些基因被分为四种不同的表达模式。KEGG通路富集表明，67个DEG参与植物激素信号通路，38个DEG参与淀粉和蔗糖代谢通路。此外，蛋白质-蛋白质相互作用网络（PPI）分析揭示了十个关键调控基因，主要参与生长素、细胞分裂素、GA、ABA和乙烯信号通路。通过定量实时PCR进一步验证了转录组数据的可靠性。总体而言，本研究为该物种不定根形成的生理和分子机制提供了新的见解，并为优化组织培养系统提供了有价值的指导。
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