酵母苯丙氨酸转运核糖核酸中紫外线诱导的交联

Behlen L S, Sampson J R, Uhlenbeck O C

Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.

Nucleic Acids Res. 1992 Aug 11;20(15):4055-9. doi: 10.1093/nar/20.15.4055.

The irradiation of native or unmodified yeast tRNA(Phe) by short wavelength UV light (260 nM) results in an intramolecular crosslink that has been mapped to occur between C48 in the variable loop and U59 in the T loop. Photo-reversibility of the crosslink and the absence of fluorescent photo adducts suggest that the crosslink product is a cytidine-uridine cyclobutane dimer. This is consistent with the relative geometries of C48 and U59 in the crystal structure of yeast tRNA(Phe). Evaluation of the crosslinking efficiency of the mutants of tRNA(Phe) indicates that the reaction depends on the correct tertiary structure of the RNA.

用短波长紫外光（260 nM）照射天然或未修饰的酵母苯丙氨酸转运核糖核酸（tRNA(Phe)）会导致分子内交联，该交联已被定位在可变环中的C48与T环中的U59之间发生。交联的光可逆性以及荧光光加合物的缺失表明交联产物是胞苷-尿苷环丁烷二聚体。这与酵母苯丙氨酸转运核糖核酸（tRNA(Phe)）晶体结构中C48和U59的相对几何结构一致。对苯丙氨酸转运核糖核酸（tRNA(Phe)）突变体交联效率的评估表明，该反应取决于RNA的正确三级结构。