York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, North Yorkshire, YO10 5YW, UK.
Nucleic Acids Res. 2010 Jul;38(12):4154-62. doi: 10.1093/nar/gkq133. Epub 2010 Mar 4.
Post-transcriptional nucleoside modifications fine-tune the biophysical and biochemical properties of transfer RNA (tRNA) so that it is optimized for participation in cellular processes. Here we report the crystal structure of unmodified tRNA(Phe) from Escherichia coli at a resolution of 3 A. We show that in the absence of modifications the overall fold of the tRNA is essentially the same as that of mature tRNA. However, there are a number of significant structural differences, such as rearrangements in a triplet base pair and a widened angle between the acceptor and anticodon stems. Contrary to previous observations, the anticodon adopts the same conformation as seen in mature tRNA.
转录后核苷修饰微调转移 RNA（tRNA）的物理化学性质，使其优化参与细胞过程。在这里，我们报道了来自大肠杆菌的未修饰 tRNA（Phe）的晶体结构，分辨率为 3 A。我们表明，在没有修饰的情况下，tRNA 的整体折叠与成熟 tRNA 基本相同。然而，存在许多显著的结构差异，例如三联体碱基对的重排和接受体与反密码子茎之间的角度增大。与先前的观察结果相反，反密码子采用与成熟 tRNA 相同的构象。
