Gillespie David K, de Peyster Ann
Graduate School of Public Health, San Diego State University, San Diego, CA, USA.
Ecotoxicol Environ Saf. 2004 May;58(1):90-5. doi: 10.1016/j.ecoenv.2003.09.005.
Numerous reports have demonstrated the vitellogenic response of fish exposed to estrogenic compounds in the aquatic environment. A strong correlation has been shown to exist between plasma vitellogenin and plasma calcium concentrations in salmonid fish. This study examines the relationship between plasma vitellogenin and calcium in a cyprinid fish, the fathead minnow (Pimephales promelas), which is a species commonly used to monitor water quality. Plasma vitellogenin was determined after exposure of male and female fish to proprietary pharmaceutical estrogens using an enzyme-linked immunosorbent assay (ELISA) developed specifically for fathead minnows. Plasma calcium was determined by flame atomic absorption spectroscopy (AA). This study demonstrated significant (P < 0.05) linear correlations between plasma vitellogenin and plasma calcium for males (r2 = 0.70), females (r2 = 0.83), and males and females combined (r2 = 0.06), showing that plasma calcium could be an indirect measure of vitellogenin present in fathead minnows. Some of the limitations and advantages identified through these studies are discussed.
众多报告表明，水生环境中接触雌激素化合物的鱼类会出现卵黄生成反应。已表明鲑科鱼类血浆中的卵黄蛋白原与血浆钙浓度之间存在密切相关性。本研究考察了鲤科鱼类黑头软口鲦（Pimephales promelas）血浆中的卵黄蛋白原与钙之间的关系，黑头软口鲦是一种常用于监测水质的物种。在雄性和雌性鱼类接触专利药用雌激素后，使用专门为黑头软口鲦开发的酶联免疫吸附测定（ELISA）法测定血浆卵黄蛋白原。通过火焰原子吸收光谱法（AA）测定血浆钙。本研究表明，雄性（r2 = 0.70）、雌性（r2 = 0.83）以及雄性和雌性合并（r2 = 0.06）的血浆卵黄蛋白原与血浆钙之间均存在显著（P < 0.05）线性相关性，这表明血浆钙可能是黑头软口鲦中卵黄蛋白原的一种间接测量指标。文中讨论了通过这些研究确定的一些局限性和优势。
