Purwaningsih I Sulistiyati, Hill Gordon A, Headley John V
Department of Chemical Engineering, Research Annex, 105 Maintenance Road, University of Saskatchewan, Saskatoon, Canada SK S7N 5C5.
Water Res. 2004 Apr;38(8):2027-34. doi: 10.1016/j.watres.2004.01.035.
Naphthalene particles in a water slurry have been bioremediated in a sealed, roller bioreactor using a pure strain of Pseudomonas putida. High stripping losses of particles due to both splashing and aeration made the use of the traditional CSTR bioreactor unsuitable for bioremediation of naphthalene particles. The overall dissolution mass transfer coefficient of naphthalene particles in the roller bioreactor was low, 0.055 h(-1) at 50 RPM. The dissolution mass transfer rate was the limiting step for bioremediation. Although mass transfer was identified as the rate limiting step, the addition of hydroxypropyl-beta-cyclodextrin (a solubility enhancer) failed to improve naphthalene slurry bioremediation. In order to successfully bioremediate naphthalene particles at concentrations over 300 mg/L, intermittent aeration was applied in the sealed roller bioreactor on a daily basis. By operating in sequential batch mode with intermittent aeration, the roller bioreactor was successfully used to continuously bioremediate naphthalene particles at concentrations up to 1000 mg/L and at rates up to 10 mg/Lh.
在一个密封的滚筒式生物反应器中，使用恶臭假单胞菌的纯菌株对水浆中的萘颗粒进行了生物修复。由于飞溅和曝气导致颗粒的高剥离损失，使得传统的连续搅拌釜式反应器不适用于萘颗粒的生物修复。在滚筒式生物反应器中，萘颗粒的总体溶解传质系数较低，在50转/分钟时为0.055 h⁻¹。溶解传质速率是生物修复的限制步骤。尽管传质被确定为速率限制步骤，但添加羟丙基-β-环糊精（一种溶解度增强剂）未能改善萘浆的生物修复。为了成功地对浓度超过300 mg/L的萘颗粒进行生物修复，每天在密封的滚筒式生物反应器中进行间歇曝气。通过采用间歇曝气的顺序分批操作模式，滚筒式生物反应器成功地用于连续生物修复浓度高达1000 mg/L、速率高达10 mg/(L·h)的萘颗粒。
