Becker Henry M, Chen Mian, Hay John B, Cybulsky Myron I
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto General Research Institute, Toronto General Hospital, 200 Elizabeth Street, Eaton-4, Toronto, Ontario, Canada M5G 2C4.
J Immunol Methods. 2004 Mar;286(1-2):69-78. doi: 10.1016/j.jim.2003.11.016.
Models of inflammatory and immune diseases are extensively studied in mice with engineered genetic mutations, and tracking the recruitment of blood leukocytes into tissues is an important component of these studies. A direct in situ method for labeling the total pool of blood cells in mice by a single intravenous injection of the fluorescent dye CFDA SE (CFSE) is described. The fluorescence intensity of labeled cells initially declines, but remains stable after 4 h, enabling detection weeks after labeling. Labeled leukocytes can be tracked as they accumulate in lymphoid tissues and sites of inflammation and then be immunophenotyped for analysis by flow cytometry. This method is rapid, reproducible and simple to perform.
炎症和免疫疾病模型在具有工程化基因突变的小鼠中得到了广泛研究，追踪血液白细胞向组织中的募集是这些研究的重要组成部分。本文描述了一种通过单次静脉注射荧光染料CFDA SE（CFSE）直接原位标记小鼠血细胞总库的方法。标记细胞的荧光强度最初会下降，但在4小时后保持稳定，从而能够在标记数周后进行检测。标记的白细胞在积聚于淋巴组织和炎症部位时可以被追踪，然后通过流式细胞术进行免疫表型分析。该方法快速、可重复且操作简单。
