Wang Xiao-Qi, Duan Xiu-Mei, Liu Li-Hua, Fang Yan-Qiu, Tan Yan
The Central Laboratory, the First Teaching Hospital of Jilin University, Changchun 130021, China.
Acta Biochim Biophys Sin (Shanghai). 2005 Jun;37(6):379-85. doi: 10.1111/j.1745-7270.2005.00051.x.
Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First, we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time. Second, we determined the optimal concentration of CFDA-SE fluorescent dye for cell labeling. Third, we tested the toxicity of CFDA-SE fluorescent dye on labeled cells. Finally, we determined the optimal staining time of CFDA-SE fluorescent dye for cell labeling. The results show that the optimal concentration of CFDA-SE fluorescent dye for cell labeling varies according to different cell types. CFDA-SE fluorescent dye is non-toxic to cells as the cell death rate caused by CFDA-SE labeling is below 5%. The optimal cell labeling time was determined to be 8 min of incubation with CFDA-SE fluorescent dye. We concluded that the advantages of using CFDA-SE fluorescent dye for cell labeling are as follows: (1) the binding of CFDA-SE fluorescent dye to cells is stable; (2) CFDA-SE fluorescent dye is not toxic and does not modify the viability of labeled cells; and (3) CFDA-SE fluorescent dye is a suitable fluorochrome for cell labeling.
我们的目的是研究羧基荧光素二乙酸琥珀酰亚胺酯（CFDA-SE）的特性以及使用CFDA-SE荧光染料进行细胞标记的方法。首先，我们分析了CFDA-SE荧光染料强度随时间的变化动力学。其次，我们确定了用于细胞标记的CFDA-SE荧光染料的最佳浓度。第三，我们测试了CFDA-SE荧光染料对标记细胞的毒性。最后，我们确定了CFDA-SE荧光染料用于细胞标记的最佳染色时间。结果表明，用于细胞标记的CFDA-SE荧光染料的最佳浓度因细胞类型而异。CFDA-SE荧光染料对细胞无毒，因为CFDA-SE标记引起的细胞死亡率低于5%。确定最佳细胞标记时间为与CFDA-SE荧光染料孵育8分钟。我们得出结论，使用CFDA-SE荧光染料进行细胞标记的优点如下：（1）CFDA-SE荧光染料与细胞的结合稳定；（2）CFDA-SE荧光染料无毒且不改变标记细胞的活力；（3）CFDA-SE荧光染料是一种适合细胞标记的荧光染料。
