使用免疫分析分析仪Architect i2000对血斑中的新生儿促甲状腺激素进行检测。
Assay of neonatal TSH from blood spots using the Architect i2000, an immunoassay analyzer.
作者信息
LePage Joyce, Peters Sherry, Lehotay Denis C
机构信息
The Provincial Laboratory, Saskatchewan, Canada S4S 5W6.
出版信息
Clin Biochem. 2004 May;37(5):415-7. doi: 10.1016/j.clinbiochem.2004.01.006.
OBJECTIVES
To assess the Architect i2000 for measuring TSH from neonatal blood spots.
METHODS
Blood collected on filter paper was eluted and assayed on the Architect.
RESULTS
Recovery of TSH was close to 100%. Within-run and between-run precisions were 4.2-8.2% and 14.5-18.1%. Correlation with the Wallac Delphia method was excellent (r = 0.96). Linearity, sensitivity, and slope were 0-400 mU/l, 0.15 mU/l, and 0.86, respectively. The results obtained by this method on the Architect allowed us to differentiate normals from affected patients with the same reliability as the previous method.
CONCLUSIONS
Measurement of TSH on the Architect is an excellent method for detecting congenital hypothyroidism.
目的
评估Architect i2000检测新生儿血斑中促甲状腺激素（TSH）的性能。
方法
将滤纸采集的血液进行洗脱，并在Architect上进行检测。
结果
TSH回收率接近100%。批内和批间精密度分别为4.2 - 8.2%和14.5 - 18.1%。与Wallac Delphia方法的相关性良好（r = 0.96）。线性范围、灵敏度和斜率分别为0 - 400 mU/l、0.15 mU/l和0.86。该方法在Architect上获得的结果使我们能够以与之前方法相同的可靠性区分正常人和患病患者。
结论
在Architect上检测TSH是检测先天性甲状腺功能减退症的一种优秀方法。
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