Wellner Edward F, Kalish Heather
Nanoscale Immunodiagnostics, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering/NIH, Bethesda, MD, USA.
Electrophoresis. 2008 Aug;29(16):3477-83. doi: 10.1002/elps.200700785.
A chip-based capillary electrophoresis system has been designed for assessing the concentrations of four hormones in whole human blood, saliva, and urine. The desired analytes were isolated by immunoextraction using a panel of four analyte-specific antibodies immobilized onto a glass fiber insert within the injection port of the chip. Following extraction, the captured analytes were labeled prior to electro-elution into the chip separation channel, where they were resolved into four individual peaks in circa 2 min. Quantification of each peak was achieved by on-line LIF detection and integration of the area under each peak. Comparison to commercial high-sensitivity immunoassays demonstrated that the chip-based assay provided fast, accurate, and precise measurements for the analytes under investigation. As the availability of commercially available antibodies rapidly expands, the application of this system will greatly increase. Chip-based CE separations of multiple analytes from a single sample also provide a significant advantage in the analysis of small samples.
一种基于芯片的毛细管电泳系统被设计用于评估全血、唾液和尿液中四种激素的浓度。通过免疫萃取分离目标分析物，使用固定在芯片进样口内玻璃纤维插入物上的一组四种分析物特异性抗体。萃取后，捕获的分析物在电洗脱进入芯片分离通道之前进行标记，在该通道中它们在大约2分钟内被分离成四个单独的峰。通过在线激光诱导荧光检测和每个峰下面积的积分实现每个峰的定量。与商业高灵敏度免疫分析方法的比较表明，基于芯片的分析方法为所研究的分析物提供了快速、准确和精确的测量。随着市售抗体的可用性迅速扩大，该系统的应用将大大增加。基于芯片的毛细管电泳从单个样品中分离多种分析物在小样品分析中也具有显著优势。
